Jenkins Paul E, Isaacs Rufus
Department of Entomology, Michigan State University, East Lansing, MI 48824, USA.
J Chem Ecol. 2008 Aug;34(8):1089-95. doi: 10.1007/s10886-008-9497-0. Epub 2008 Jun 26.
A paraffin wax formulation releasing pheromone for mating disruption of insects was tested during 2005 and 2006 in Vitis labrusca vineyards infested by grape berry moth, Paralobesia viteana (Lepidoptera: Tortricidae). In early May of each year, 1-ml droplets of SPLAT-GBM wax containing 3% sex pheromone of P. viteana were applied to every wooden post at a rate of 400/ha in replicated 1.3-ha plots. Moth captures in sex pheromone baited traps placed at the vineyard borders and interiors revealed significant disruption of male moth captures in treated plots, with activity of one application lasting over 10 weeks during both years. Treatment with SPLAT-GBM did not affect the proportion of clusters infested until the end of the second growing season, when infestation was 27% lower in the treated plots than in the control plots. Comparisons of moth captures in traps placed inside 15.2 x 16.5 m vine plots that were untreated or received varying densities of 0.2-ml wax drops or Isomate-GBM hand-tied dispensers at the recommended rate of 450/ha indicated that orientational disruption increased with droplet density. Similar numbers of moths were captured in plots that received 10 or 30 drops per vine as were trapped in plots with twist ties spaced at 0.4 per vine. Moth captures in monitoring traps baited with increasing sizes of wax droplets (0.2, 0.5, or 1-ml drops) or red septa containing P. viteana sex pheromone suggest decreasing ability of male moths to reach traps with increasing pheromone loading. This study indicates that wax-deployed pheromone can reduce crop infestation by P. viteana after 2 years of deployment, and that the increasing of pheromone release by using application of greater droplet densities or by using larger droplets will improve the level of disruption achieved.
一种用于干扰昆虫交配的释放性信息素的石蜡配方,于2005年和2006年在受葡萄浆果小卷蛾(Paralobesia viteana,鳞翅目:卷蛾科)侵害的美洲葡萄园中进行了测试。每年5月初,将含有3%葡萄浆果小卷蛾性信息素的SPLAT-GBM蜡的1毫升液滴,以400个/公顷的速率施用于每个木桩上,设置在1.3公顷的重复地块中。放置在葡萄园边界和内部的性信息素诱捕器捕获的蛾子显示,处理地块中雄性蛾子的捕获量受到显著干扰,两年中一次施用的效果持续超过10周。直到第二个生长季节结束,SPLAT-GBM处理才对受侵染的果穗比例产生影响,此时处理地块中的侵染率比对照地块低27%。对放置在15.2×16.5米葡萄园内未经处理或接受不同密度0.2毫升蜡滴或按450个/公顷推荐速率使用的Isomate-GBM手工系挂式诱捕器的地块中诱捕到的蛾子进行比较,结果表明,定向干扰随液滴密度的增加而增加。每株葡萄接受10或30滴的地块中捕获的蛾子数量,与每株葡萄间隔0.4个扭结带的地块中捕获的蛾子数量相似。用不同大小的蜡滴(0.2、0.5或1毫升液滴)或含有葡萄浆果小卷蛾性信息素的红色隔片作为诱饵的监测诱捕器捕获的蛾子表明,随着性信息素负载量的增加,雄性蛾子到达诱捕器的能力下降。这项研究表明,经过两年的施用,蜡质剂型的性信息素可以减少葡萄浆果小卷蛾对作物的侵害,并且通过施用更大的液滴密度或更大的液滴来增加性信息素的释放,将提高干扰水平。