在固定化酵母膜生物反应器中用重组酵母将乳糖发酵为乙醇。
Fermentation of lactose to ethanol with recombinant yeast in an immobilized yeast membrane bioreactor.
作者信息
Jeong Y S, Vieth W R, Matsuura T
机构信息
Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, New Jersey 08855-0909, USA.
出版信息
Biotechnol Bioeng. 1991 Mar 15;37(6):587-90. doi: 10.1002/bit.260370614.
A novel concept of membrane bioreactor in which living cells are sandwiched between ultrafiltration (UF) and reverse osmosis (RO) membranes was applied for lactose fermentation to ethanol by genetically engineered yeast cells. The productivity of the Lactophile 13B strains was higher than that of the Lactophile 13D strains. In both cases performance data similar to those for glucose fermentation to ethanol by Saccharomyces cerevisiae were obtained. However, the operational stability of recombinant yeast cells was improved in the new bioreactor in comparison to the stability of these cells in a shake flask.
一种新型的膜生物反应器概念，即将活细胞夹在超滤（UF）膜和反渗透（RO）膜之间，被应用于通过基因工程酵母细胞将乳糖发酵为乙醇。嗜乳糖13B菌株的生产效率高于嗜乳糖13D菌株。在这两种情况下，都获得了与酿酒酵母将葡萄糖发酵为乙醇时相似的性能数据。然而，与摇瓶中这些细胞的稳定性相比，重组酵母细胞在新型生物反应器中的操作稳定性得到了提高。
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