Lee Hakho, Sun Eric, Ham Donhee, Weissleder Ralph
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
Nat Med. 2008 Aug;14(8):869-74. doi: 10.1038/nm.1711. Epub 2008 Jul 6.
Rapid and accurate measurement of biomarkers in tissue and fluid samples is a major challenge in medicine. Here we report the development of a new, miniaturized diagnostic magnetic resonance (DMR) system for multiplexed, quantitative and rapid analysis. By using magnetic particles as a proximity sensor to amplify molecular interactions, the handheld DMR system can perform measurements on unprocessed biological samples. We show the capability of the DMR system by using it to detect bacteria with high sensitivity, identify small numbers of cells and analyze them on a molecular level in real time, and measure a series of protein biomarkers in parallel. The DMR technology shows promise as a robust and portable diagnostic device.
在组织和液体样本中快速准确地测量生物标志物是医学领域的一项重大挑战。在此,我们报告了一种新型小型化诊断磁共振(DMR)系统的开发,用于多重、定量和快速分析。通过使用磁性颗粒作为邻近传感器来放大分子相互作用,手持式DMR系统可以对未处理的生物样本进行测量。我们展示了DMR系统的能力,即利用它以高灵敏度检测细菌、识别少量细胞并实时在分子水平上对其进行分析,以及并行测量一系列蛋白质生物标志物。DMR技术有望成为一种强大且便携的诊断设备。