Pedersen A R, Møller S, Molin S, Arvin E
Institute of Environmental Science and Engineering, The Technical University of Denmark, Building 115, DK-2800 Lyngby, Denmark.
Biotechnol Bioeng. 1997 Apr 20;54(2):131-41. doi: 10.1002/(SICI)1097-0290(19970420)54:2<131::AID-BIT5>3.0.CO;2-M.
A biological trickling filter for treatment of toluene-containing waste gas was studied. The overall kinetics of the biofilm growth was followed in the early growth phase. A rapid initial colonization took place during the first three days. The biofilm thickness increased exponentially, whereas the incease of active biomass and polymers was linear. In order to investigate the toluene degradation, various toluene degraders from the multispecies biofilm were isolated, and a Pseudomonas putida was chosen as a representative of the toluene-degrading population. A specific rRNA oligonucleotide probe was used to follow the toluene-degrading P. putida in the multispecies biofilm in the filter by means of number and cellular rRNA content. P. putida appeared to detach from the biofilm during the first three days of growth, after which P. putida was found at a constant level of 10% of the active biomass in the biofilm. Based on the rRNA content, the in situ activity was estimated to be reduced to 20% of cells grown at maximum conditions in batch culture. The toluene degraded by P. putida was estimated to be a minor part (11%) of the overall toluene degradation.
研究了一种用于处理含甲苯废气的生物滴滤器。在生物膜生长的早期阶段跟踪其生长的整体动力学。在最初的三天内发生了快速的初始定殖。生物膜厚度呈指数增长，而活性生物量和聚合物的增加呈线性。为了研究甲苯降解情况，从多物种生物膜中分离出各种甲苯降解菌，并选择恶臭假单胞菌作为甲苯降解菌群的代表。使用一种特定的rRNA寡核苷酸探针，通过数量和细胞rRNA含量来跟踪过滤器中多物种生物膜里降解甲苯的恶臭假单胞菌。恶臭假单胞菌在生长的头三天似乎从生物膜上脱离，此后在生物膜中发现恶臭假单胞菌的含量稳定在活性生物量的10%。根据rRNA含量，原位活性估计降至在分批培养中最大条件下生长的细胞的20%。恶臭假单胞菌降解的甲苯估计仅占甲苯总降解量的一小部分（11%）。
