一种商用酶联免疫吸附测定法可检测到高水平的人H5抗体，但会与甲型流感抗体发生交叉反应。
A commercial ELISA detects high levels of human H5 antibody but cross-reacts with influenza A antibodies.
作者信息
Stelzer-Braid Sacha, Wong Bruce, Robertson Peter, Lynch Garry W, Laurie Karen, Shaw Robert, Barr Ian, Selleck Paul W, Baleriola Cristina, Escott Ros, Katsoulotos Gregory, Rawlinson William D
机构信息
Virology Research, Department of Microbiology, South Eastern Area Laboratory Service, Prince of Wales Hospital, Randwick, NSW 2031, Australia.
出版信息
J Clin Virol. 2008 Oct;43(2):241-3. doi: 10.1016/j.jcv.2008.06.012. Epub 2008 Aug 3.
BACKGROUND AND OBJECTIVES
Commercial serological assays to determine influenza A H5N1 infection are available, although the accuracy and reproducibility of these are not reported in detail. This study aimed to assess the validity of a commercial ELISA H5 hemagglutinin (HA) antibody kit.
STUDY DESIGN
A commercial ELISA for detection of antibodies towards influenza A H5 HA was evaluated using human sera from vaccinated individuals. The ELISA was used to screen 304 sera with elevated influenza A complement fixation titres collected between the period 1995-2007.
RESULTS AND CONCLUSIONS
The ELISA was found to be accurate for sera with high levels of anti-H5 antibodies, and would be useful in clinical settings where a rapid result is required. Thirteen of the stored sera were positive using the ELISA, but were confirmed as negative for H5N1 exposure using further serological tests. Absorption studies suggested that antibodies towards seasonal H3N2 and H1N1 influenza may cross-react with H5 antigen, giving false positive results with the ELISA.
背景与目的
用于检测甲型H5N1流感感染的商业血清学检测方法已有可用，但这些方法的准确性和可重复性尚未详细报道。本研究旨在评估一种商业ELISA H5血凝素（HA）抗体试剂盒的有效性。
研究设计
使用来自接种疫苗个体的人血清评估一种用于检测甲型H5 HA抗体的商业ELISA。该ELISA用于筛查1995年至2007年期间收集的304份甲型流感补体结合试验滴度升高的血清。
结果与结论
发现该ELISA对于抗H5抗体水平高的血清是准确的，并且在需要快速结果的临床环境中会有用。使用该ELISA检测，13份储存的血清呈阳性，但通过进一步的血清学检测确认这些血清未接触过H5N1。吸收研究表明，针对季节性H3N2和H1N1流感的抗体可能与H5抗原发生交叉反应，导致ELISA出现假阳性结果。
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