哥斯达黎加一家医院成年医院获得性腹泻患者中的艰难梭菌
Clostridium difficile in adult patients with nosocomial diarrhea in a Costa Rican hospital.
作者信息
Zumbado-Salas Roberto, Gamboa-Coronado María del Mar, Rodríguez-Cavallini Evelyn, Chaves-Olarte Esteban
机构信息
Laboratorio de Investigación en Bacteriología Anaerobia, Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, San José, Costa Rica.
出版信息
Am J Trop Med Hyg. 2008 Aug;79(2):164-5.
Stool samples from 104 adult patients with nosocomial antibiotic-associated diarrhea were analyzed for Clostridium difficile by cultivation, toxin A inmunoenzymatic detection, and toxin B cytotoxic detection. The isolates were additionally screened for the toxin genes by polymerase chain reaction. C. difficile was isolated from 26 samples, and the toxins were directly detected in another 5 samples. Toxin A and B genes were detected in all toxigenic bacterial isolates. The detection rate of 30% indicates that C. difficile is a major etiologic agent of nosocomial diarrhea in Costa Rica.
对104例成人医院获得性抗生素相关性腹泻患者的粪便样本进行艰难梭菌分析，采用培养法、毒素A免疫酶检测法和毒素B细胞毒性检测法。此外，通过聚合酶链反应对分离株进行毒素基因筛查。从26份样本中分离出艰难梭菌，另外在5份样本中直接检测到毒素。在所有产毒细菌分离株中均检测到毒素A和B基因。30%的检出率表明艰难梭菌是哥斯达黎加医院感染性腹泻的主要病原体。
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