稻瘟病菌稻瘟病菌微卫星标记的开发及遗传图谱的构建

Development of microsatellite markers and construction of genetic map in rice blast pathogen Magnaporthe grisea.

作者信息

Zheng Yan, Zhang Geng, Lin Fucheng, Wang Zonghua, Jin Gulei, Yang Long, Wang Ying, Chen Xi, Xu Zhaohua, Zhao Xiangqian, Wang Hongkai, Lu Jianping, Lu Guodong, Wu Weiren

机构信息

Department of Agronomy, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, China.

出版信息

Fungal Genet Biol. 2008 Oct;45(10):1340-7. doi: 10.1016/j.fgb.2008.07.012. Epub 2008 Jul 24.

DOI:10.1016/j.fgb.2008.07.012

PMID:18694839

Abstract

Magnaporthe grisea is the most destructive fungal pathogen of rice and a model organism for studying plant-pathogen interaction. Molecular markers and genetic maps are useful tools for genetic studies. In this study, based on the released genome sequence data of M. grisea, we investigated 446 simple sequence repeat (SSR) loci and developed 313 SSR markers, which showed polymorphisms among nine isolates from rice (including a laboratory strain 2539). The number of alleles of each marker ranged 2-9 with an average of 3.3. The polymorphic information content (PIC) of each marker ranged 0.20-0.89 with an average of 0.53. Using a population derived from a cross between isolates Guy11 and 2539, we constructed a genetic map of M. grisea consisting of 176 SSR markers. The map covers a total length of 1247 cM, equivalent to a physical length of about 35.0 Mb or 93% of the genome, with an average distance of 7.1cM between adjacent markers. A web-based database of the SSR markers and the genetic map was established (http://ibi.zju.edu.cn/pgl/MGM/index.html).

摘要

稻瘟病菌是水稻最具破坏性的真菌病原体，也是研究植物与病原体相互作用的模式生物。分子标记和遗传图谱是遗传学研究的有用工具。在本研究中，基于已发布的稻瘟病菌基因组序列数据，我们研究了446个简单序列重复（SSR）位点，并开发了313个SSR标记，这些标记在来自水稻的9个分离株（包括实验室菌株2539）中表现出多态性。每个标记的等位基因数量在2至9个之间，平均为3.3个。每个标记的多态信息含量（PIC）在0.20至0.89之间，平均为0.53。利用分离株Guy11和2539杂交产生的群体，我们构建了一张由176个SSR标记组成的稻瘟病菌遗传图谱。该图谱全长1247厘摩，相当于约35.0兆碱基的物理长度或基因组的93%，相邻标记之间的平均距离为7.1厘摩。建立了一个基于网络的SSR标记和遗传图谱数据库（http://ibi.zju.edu.cn/pgl/MGM/index.html）。