用于定量生物光谱分析的本征拉曼光谱学 第一部分：理论与模拟
Intrinsic Raman spectroscopy for quantitative biological spectroscopy part I: theory and simulations.
作者信息
Shih Wei-Chuan, Bechtel Kate L, Feld Michael S
机构信息
GR Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
出版信息
Opt Express. 2008 Aug 18;16(17):12726-36. doi: 10.1364/oe.16.012726.
Abstract
We present a novel technique, intrinsic Raman spectroscopy (IRS), to correct turbidity-induced Raman spectral distortions, resulting in the intrinsic Raman spectrum that would be observed in the absence of scattering and absorption. We develop an expression relating the observed and intrinsic Raman spectra through diffuse reflectance using the photon migration depiction of light transport. Numerical simulations are employed to validate the theoretical results and study the dependence of this expression on sample size and elastic scattering anisotropy.
摘要
我们提出了一种新技术——本征拉曼光谱法（IRS），用于校正浊度引起的拉曼光谱失真，从而得到在不存在散射和吸收情况下会观察到的本征拉曼光谱。我们利用光传输的光子迁移描述，通过漫反射建立了一个将观察到的拉曼光谱与本征拉曼光谱相关联的表达式。采用数值模拟来验证理论结果，并研究该表达式对样品尺寸和弹性散射各向异性的依赖性。
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