牛乳腺上皮细胞系（BME-UV1）代谢活性的研究。

Investigation of the metabolic activity of a bovine mammary epithelial cell line (BME-UV1).

作者信息

Caruso M, Belloli C, Zaghini A, Altafini A, Ormas P

机构信息

Department of Veterinary Public Health, University of Bari, Strada prov. per Casamassima km 3, Valenzano 70010, Bari, Italy.

出版信息

Vet Res Commun. 2008 Sep;32 Suppl 1:S259-62. doi: 10.1007/s11259-008-9123-1.

DOI:10.1007/s11259-008-9123-1

PMID:18712616

Abstract

The metabolic activity of a mammary epithelial cell line (BME-UV1) was evaluated on monolayers exposed, in serum free medium, to different concentrations (2-4-8 muM) of aflatoxin B1 (AFB1), a mycotoxin eliminated into milk especially as hydroxylated metabolite aflatoxin M1 (AFM1). After 4, 8, 12, 24 h of treatment, a dose and time dependent production of AFM1 has been detected. As the enzymes involved in the hydroxylation of AFB1 in bovine hepatocytes are mainly CYP1A and CYP3A, the results suggest that BME-UV1 express CYP450 isoenzymes which metabolize AFB1 thus representing a potential model for the investigation of the metabolic activity of bovine mammary epithelial tissue.

摘要

在无血清培养基中，将乳腺上皮细胞系（BME-UV1）单层暴露于不同浓度（2-4-8微摩尔）的黄曲霉毒素B1（AFB1）下，评估其代谢活性。黄曲霉毒素B1是一种霉菌毒素，尤其以羟基化代谢物黄曲霉毒素M1（AFM1）的形式进入牛奶。处理4、8、12、24小时后，检测到AFM1的产生呈剂量和时间依赖性。由于牛肝细胞中参与AFB1羟基化的酶主要是CYP1A和CYP3A，结果表明BME-UV1表达代谢AFB1的CYP450同工酶，因此代表了研究牛乳腺上皮组织代谢活性的潜在模型。