猪肿瘤坏死因子编码基因：序列与比较分析。
The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis.
作者信息
Kuhnert P, Wüthrich C, Peterhans E, Pauli U
机构信息
Institute of Veterinary Virology, University of Berne, Switzerland.
出版信息
Gene. 1991 Jun 30;102(2):171-8. doi: 10.1016/0378-1119(91)90075-m.
We have cloned and sequenced a 10.22-kb fragment of the genomic locus of the porcine tumor necrosis factor-encoding genes, TNF-alpha and TNF-beta. A liver genomic DNA library, partially digested with Sau3AI, was cloned into the phage lambda EMBL4 and screened with a porcine TNF-alpha cDNA probe. Analysis showed that both the TNF-alpha and TNF-beta genes were present on the cloned fragment. In addition, the cloned fragment contained about 2 kb of repetitive sequences 5' to the TNF-beta gene. The TNF genes are arranged in a tandem repeat, as is the case for the human, mouse and rabbit TNF genes. The comparison of both genes with their human homologues displayed a considerable degree of conservation (80%), suggesting an equal evolution rate.
我们已克隆并测序了猪肿瘤坏死因子编码基因TNF-α和TNF-β基因组位点的一个10.22kb片段。用Sau3AI部分消化的肝脏基因组DNA文库被克隆到噬菌体λEMBL4中，并用猪TNF-α cDNA探针进行筛选。分析表明，克隆片段上同时存在TNF-α和TNF-β基因。此外，克隆片段在TNF-β基因5'端含有约2kb的重复序列。TNF基因呈串联重复排列，人类、小鼠和兔的TNF基因也是如此。这两个基因与其人类同源物的比较显示出相当程度的保守性（80%），表明进化速率相同。
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