三种探针在耐甲氧西林金黄色葡萄球菌（MRSA）分离株分型中的应用价值。

Usefulness of three probes in typing isolates of methicillin-resistant Staphylococcus aureus (MRSA).

作者信息

Monzon-Moreno C, Aubert S, Morvan A, Solh N E

机构信息

Laboratoire des Staphylocoques et des Streptocoques, National Reference Center for Staphylococci, Institut Pasteur, Paris, France.

出版信息

J Med Microbiol. 1991 Aug;35(2):80-8. doi: 10.1099/00222615-35-2-80.

DOI:10.1099/00222615-35-2-80

PMID:1875395

Abstract

Fifty-nine epidemiologically unrelated methicillin-resistant Staphylococcus aureus (MRSA) isolates from different geographical areas and 23 phage-type 77 MRSA isolates from France were investigated. Cellular DNA, digested with restriction endonucleases EcoRI or HindIII, was probed with plasmids carrying the gene encoding 16S rRNA (pBA2), the gene aacA-aphD (pSF815A) and the gene aacA-aphD plus part of IS256 (pIP1307). When probed with pBA2, most of the unrelated isolates displayed the same hybridisation pattern. A greater diversity in patterns was detected in gentamicin-resistant strains with the two other probes. The most accurate fingerprinting of these isolates was obtained with the probe pIP1307. Moreover, this probe appeared to be useful for tracing the phage-type 77 epidemic MRSA isolates widespread in French hospitals.

摘要

对来自不同地理区域的59株流行病学上不相关的耐甲氧西林金黄色葡萄球菌（MRSA）分离株以及来自法国的23株噬菌体77型MRSA分离株进行了研究。用限制性内切酶EcoRI或HindIII消化细胞DNA，并用携带编码16S rRNA的基因（pBA2）、aacA-aphD基因（pSF815A）以及aacA-aphD基因加部分IS256（pIP1307）的质粒进行杂交检测。用pBA2进行检测时，大多数不相关的分离株显示出相同的杂交模式。用另外两种探针检测耐庆大霉素菌株时，检测到的模式多样性更大。用探针pIP1307获得了这些分离株最准确的指纹图谱。此外，该探针似乎有助于追踪在法国医院广泛传播的噬菌体77型流行MRSA分离株。