Reena Rahayu Md Zin, Mastura Mokhtar, Siti-Aishah Md Ali, Munirah Md Ali, Norlia Abdullah, Naqiyah Ibrahim, Rohaizak Muhamad, Sharifah Noor Akmal
Department of Pathology, Hospital Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000 Cheras Kuala Lumpur, Malaysia.
Ann Diagn Pathol. 2008 Oct;12(5):340-3. doi: 10.1016/j.anndiagpath.2008.04.001. Epub 2008 Jul 7.
This is a study aimed to examine the distribution pattern of a specific minichromosome maintenance protein 2 (MCM2) in benign and malignant breast tissue. We also aim to correlate the frequency of expression of MCM2 with the degree of tumor differentiation. We used immunohistochemistry to examine the distribution and expression pattern of MCM2 on formalin-fixed paraffin-embedded tissue sections of benign (n = 30) and malignant breast tissue (n = 70) (IDC 56, DCIS 4, ILC 2, nonductal 4, mixed type 4). We quantified MCM2 expression by calculating a labeling index, which represents the percentage of epithelial nuclei that stained positively. Immunoreactivity was heterogenous in all the 70 malignant cases examined. Epithelial cells in cycle are most frequent at the tumor periphery. Labeling index of MCM2 was greatest in grade 3 (poorly differentiated) and lowest in grade 1 tumors (well differentiated). Minichromosome maintenance protein 2 expression in breast cancer showed a positive association with histologic grade (P < .05). In all the benign breast tissue examined, no proliferating compartments could be characterized. Minichromosome maintenance protein 2 is a useful proliferative marker of breast carcinoma. The frequency of expression of MCM2 showed an inverse correlation with the degree of tumor differentiation.
这是一项旨在研究特定微小染色体维持蛋白2（MCM2）在乳腺良性和恶性组织中分布模式的研究。我们还旨在将MCM2的表达频率与肿瘤分化程度相关联。我们采用免疫组织化学方法检测MCM2在良性乳腺组织（n = 30）和恶性乳腺组织（n = 70）（浸润性导管癌56例、导管原位癌4例、浸润性小叶癌2例、非导管癌4例、混合型4例）的福尔马林固定石蜡包埋组织切片上的分布及表达模式。我们通过计算标记指数来量化MCM2的表达，该指数代表阳性染色上皮细胞核的百分比。在所检测的70例恶性病例中，免疫反应性是异质性的。处于细胞周期的上皮细胞在肿瘤周边最为常见。MCM2的标记指数在3级（低分化）肿瘤中最高，在1级（高分化）肿瘤中最低。乳腺癌中微小染色体维持蛋白2的表达与组织学分级呈正相关（P < 0.05）。在所有检测的良性乳腺组织中，未发现增殖区室。微小染色体维持蛋白2是乳腺癌有用的增殖标志物。MCM2的表达频率与肿瘤分化程度呈负相关。
