通过2'-O,4'-C-氨基亚甲基桥连核酸（2',4'-BNA NC）修饰促进三链体形成。

Promotion of triplex formation by 2'-O,4'-C-aminomethylene bridged nucleic acid (2',4'-BNA NC) modification.

作者信息

Sasaki Kiyomi, Rahman S M Abdur, Obika Satoshi, Imanishi Takeshi, Torigoe Hidetaka

机构信息

Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, Shinjuku-ku, Tokyo 162-8601, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):419-20. doi: 10.1093/nass/nrn213.

DOI:10.1093/nass/nrn213

PMID:18776432

Abstract

We examined the effect of 2'-O,4'-C-aminomethylene bridged nucleic acid (2',4'-BNA(NC)) backbone modification of triplex-forming oligonucleotide (TFO) on the pyrimidine motif triplex formation at neutral pH, a condition where pyrimidine motif triplexes are unstable. The melting temperature of the pyrimidine motif triplex at pH 6.8 with 2',4'-BNA(NC) modified TFO was significantly higher than that observed with unmodified TFO. The 2',4'-BNA(NC) modification of TFO increased the thermal stability of the pyrimidine motif triplex at neutral pH. The present results certainly support the idea that the 2',4'-BNA(NC) backbone modification of TFO could be a key chemical modification and may eventually lead to progress in therapeutic applications of the antigene strategy in vivo.

摘要

我们研究了三链形成寡核苷酸（TFO）的2'-O，4'-C-氨基亚甲基桥连核酸（2'，4'-BNA(NC)）主链修饰对中性pH条件下嘧啶基序三链形成的影响，在该条件下嘧啶基序三链不稳定。在pH 6.8时，2'，4'-BNA(NC)修饰的TFO形成的嘧啶基序三链的解链温度显著高于未修饰TFO形成的三链。TFO的2'，4'-BNA(NC)修饰提高了中性pH条件下嘧啶基序三链的热稳定性。目前的结果确实支持这样一种观点，即TFO的2'，4'-BNA(NC)主链修饰可能是一种关键的化学修饰，并最终可能推动体内反基因策略治疗应用的进展。