Mui Timothy P, Silverman Scott K
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Org Lett. 2008 Oct 16;10(20):4417-20. doi: 10.1021/ol801568q. Epub 2008 Sep 23.
We report a deoxyribozyme (DNA enzyme) that catalyzes the convergent and general synthesis of branched DNA. The 15HA9 deoxyribozyme mediates nucleophilic attack of the 2'-hydroxyl group of a ribonucleotide embedded within one DNA substrate into a 5'-adenylate of the second DNA substrate. This approach can be used to synthesize multiply branched DNA with a wide range of DNA sequences.
我们报道了一种催化分支DNA收敛性和通用合成的脱氧核酶(DNA酶)。15HA9脱氧核酶介导嵌入一个DNA底物中的核糖核苷酸的2'-羟基对第二个DNA底物的5'-腺苷酸进行亲核攻击。这种方法可用于合成具有广泛DNA序列的多重分支DNA。