大鼠脑微血管溶质通透性的无创测量
Non-invasive measurement of solute permeability in cerebral microvessels of the rat.
作者信息
Yuan Wei, Lv Yonggang, Zeng Min, Fu Bingmei M
机构信息
Department of Biomedical Engineering, The City College of the City University of New York, 138th Street at Convent Avenue, New York, NY 10031, USA.
出版信息
Microvasc Res. 2009 Mar;77(2):166-73. doi: 10.1016/j.mvr.2008.08.004. Epub 2008 Sep 11.
To quantify the solute permeability of rat cerebral microvessels, we measured the apparent permeability (P) of pial microvessels to various sized solutes. The pial microcirculation was observed by a high numerical aperture objective lens through a section of the frontoparietal bone thinned with a micro-grinder (a revised method from Easton and Fraser, 1994, J Physiol. 475:147-157, 1994). Sodium fluorescein (MW 376) at concentration 0.1 mg/ml or FITC-dextrans (MW 4k, 10k, 20k, 40k, 70k) at concentration 1 mg/ml in 1% BSA mammalian Ringer, was introduced into the cerebral circulation via the ipsilateral carotid artery by a syringe pump at a constant rate of approximately 3 ml/min. P was determined using a highly sensitive quantitative fluorescence imaging and analyzing method. The mean P to sodium fluorescein was 2.71 (+/-0.76 SD, n=11)x10(-6) cm/s. The mean P to FITC-dextrans were 0.92 (+/-0.46 SD, n=10)x10(-6) cm/s for Dextran-4k, 0.31 (+/-0.13 SD, n=7)x10(-6) cm/s for Dextran-10k, 0.24 (+/-0.10 SD, n=6)x10(-6) cm/s for Dextran-20k, 0.19 (+/-0.11 SD, n=10)x10(-6) cm/s for Dextran-40k, and 0.15 (+/-0.05 SD, n=11)x10(-6) cm/s for Dextran-70k. These values were 1/10 to 1/6 of those of rat mesenteric microvessels for similar sized solutes (Fu, B.M., Shen, S., 2004. Acute VEGF effect on solute permeability of mammalian microvessels in vivo. Microvasc. Res. 68, 51-62.).
为了量化大鼠脑微血管的溶质通透性，我们测量了软脑膜微血管对各种大小溶质的表观通透性（P）。通过高数值孔径物镜，透过用微型磨床磨薄的额顶骨切片观察软脑膜微循环（这是对Easton和Fraser于1994年发表在《生理学杂志》上的方法的改进，该杂志第475卷，第147 - 157页，1994年）。将浓度为0.1mg/ml的荧光素钠（分子量376）或浓度为1mg/ml的异硫氰酸荧光素 - 葡聚糖（分子量4k、10k、20k、40k、70k）溶于1%牛血清白蛋白的哺乳动物林格氏液中，通过注射泵以约3ml/min的恒定速率经同侧颈动脉注入脑循环。使用高灵敏度的定量荧光成像和分析方法测定P值。荧光素钠的平均P值为2.71（±0.76标准差，n = 11）×10⁻⁶cm/s。异硫氰酸荧光素 - 葡聚糖的平均P值分别为：葡聚糖 - 分子量4k的为0.92（±0.46标准差，n = 10）×10⁻⁶cm/s，葡聚糖 - 分子量10k的为0.31（±0.13标准差，n = 7）×10⁻⁶cm/s，葡聚糖 - 分子量20k的为0.24（±0.10标准差，n = 6）×10⁻⁶cm/s，葡聚糖 - 分子量40k的为0.19（±0.11标准差，n = 10）×10⁻⁶cm/s，葡聚糖 - 分子量70k的为0.15（±0.05标准差，n = 11）×10⁻⁶cm/s。对于类似大小的溶质，这些值是大鼠肠系膜微血管相应值的1/10至1/6（Fu，B.M.，Shen，S.，2004年。急性血管内皮生长因子对哺乳动物体内微血管溶质通透性的影响。微血管研究。第68卷，第51 - 62页）。
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