Gupta P, Farzadegan H, Imagawa D, Lee M, Kingsley L, Zhou S, Armstrong J, Rinaldo C
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
J Clin Microbiol. 1991 Jul;29(7):1368-71. doi: 10.1128/jcm.29.7.1368-1371.1991.
A prospective multicenter study was undertaken to isolate human immunodeficiency virus type 1 (HIV-1) from 45 homosexual men for a period of 30 months after seroconversion. Efficiency of HIV-1 isolation from peripheral blood mononuclear cells was relatively stable over time, ranging from 64% at the time of seroconversion to more than 82% after 18 months of seroconversion. However, Kaplan-Meier analysis of HIV-1 culture data indicates that the cumulative proportion of HIV-1 culture positivity at 3, 6, 12, and 18 months after seroconversion was 62, 65, 84, and 92%, respectively. No significant correlation was observed between the presence of HIV-1 p24 antigen in serum, or numbers of CD4+ and CD8+ blood lymphocytes, and HIV-1 isolation within this period of time. These data suggest that HIV-1 viremia in homosexual men gradually increases to almost 100% culture positivity by 18 months after seroconversion.
一项前瞻性多中心研究对45名同性恋男性在血清转化后30个月内进行了1型人类免疫缺陷病毒(HIV-1)分离。从外周血单个核细胞中分离HIV-1的效率随时间相对稳定,血清转化时为64%,血清转化18个月后超过82%。然而,对HIV-1培养数据的Kaplan-Meier分析表明,血清转化后3、6、12和18个月时HIV-1培养阳性的累积比例分别为62%、65%、84%和92%。在此时间段内,血清中HIV-1 p24抗原的存在、CD4+和CD8+血淋巴细胞数量与HIV-1分离之间未观察到显著相关性。这些数据表明,同性恋男性中的HIV-1病毒血症在血清转化后18个月时逐渐增加至几乎100%的培养阳性率。