研究亚型特异性肌醇1,4,5-三磷酸受体的功能与调控。
Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation.
作者信息
Betzenhauser Matthew J, Wagner Larry E, Won Jong Hak, Yule David I
机构信息
Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA.
出版信息
Methods. 2008 Nov;46(3):177-82. doi: 10.1016/j.ymeth.2008.09.014. Epub 2008 Oct 16.
Abstract
Inositol 1,4,5-trisphosphate receptors (InsP3R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP3R family members with particular reference to methods applicable following expression of recombinant InsP3R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP3R expression system.
摘要
肌醇1,4,5-三磷酸受体（InsP3R）是一类广泛表达的细胞内钙离子通道家族。三个家族成员的亚型特异性特性可能在决定细胞内钙离子信号时空特征的丰富多样性方面发挥重要作用。研究特定家族成员的特性很复杂，因为单个受体亚型通常不会单独表达。在本文中，我们讨论了通过单个InsP3R家族成员研究钙离子释放的策略，特别提及了在DT40-3KO细胞系（一种明确的InsP3R缺失表达系统）中表达重组InsP3R和突变构建体后适用的方法。
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