重组促红细胞生成素、粒细胞-巨噬细胞集落刺激因子、白细胞介素3和白细胞介素6对纯化大鼠巨核细胞的生物学效应。
Biological effects of recombinant erythropoietin, granulocyte-macrophage colony-stimulating factor, interleukin 3, and interleukin 6 on purified rat megakaryocytes.
作者信息
Ishida Y, Yano S, Yoshida T, Tanaka H, Yamada Y, Kawano M, Kaneko T, Matsumoto N
机构信息
Third Department of Internal Medicine, Yamaguchi University School of Medicine, Japan.
出版信息
Exp Hematol. 1991 Aug;19(7):608-12.
The biological effects of recombinant hematopoietic factors, which are considered to stimulate megakaryocytopoiesis in vitro or in vivo, were studied utilizing purified rat megakaryocytes. Recombinant human erythropoietin (rhEPO), recombinant murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF), and recombinant murine interleukin 3 (rmIL-3) stimulated both [3H]thymidine and [3H]leucine incorporation into purified rat megakaryocytes. In contrast, recombinant human interleukin 6 (rhIL-6) did not stimulate [3H]thymidine uptake but did increase [3H]leucine incorporation into purified rat megakaryocytes. These in vitro data suggest that DNA synthesis in megakaryocytes may be regulated by EPO, GM-CSF, and IL-3, and that protein synthesis is stimulated by EPO, GM-CSF, IL-3, and IL-6 in vitro.
利用纯化的大鼠巨核细胞研究了重组造血因子的生物学效应，这些因子被认为可在体外或体内刺激巨核细胞生成。重组人促红细胞生成素（rhEPO）、重组小鼠粒细胞 - 巨噬细胞集落刺激因子（rmGM - CSF）和重组小鼠白细胞介素3（rmIL - 3）均刺激[³H]胸苷和[³H]亮氨酸掺入纯化的大鼠巨核细胞。相比之下，重组人白细胞介素6（rhIL - 6）不刺激[³H]胸苷摄取，但确实增加[³H]亮氨酸掺入纯化的大鼠巨核细胞。这些体外数据表明，巨核细胞中的DNA合成可能受EPO、GM - CSF和IL - 3调节，并且体外蛋白质合成受EPO、GM - CSF、IL - 3和IL - 6刺激。
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