苏云金芽孢杆菌菌株YBT-1520隐蔽质粒上两个假定的毒素-抗毒素系统的遗传特征分析
Genetic characterization of two putative toxin-antitoxin systems on cryptic plasmids from Bacillus thuringiensis strain YBT-1520.
作者信息
Liu Xiaojin, Zhu Shufang, Ye Weixing, Ruan Lifang, Yu Ziniu, Zhao Changming, Sun Ming
机构信息
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University,430070 Wuhan, P R China.
出版信息
J Microbiol Biotechnol. 2008 Oct;18(10):1630-3.
A novel putative toxin-antitoxin segregational stability system named KyAB system was identified in a novel native plasmid pBMB8240 from Bacillus thuringiensis strain YBT-1520, based on sequences homology with other toxin-antitoxin systems, the lethal activity of the KyB putative toxin in Escherichia coli and the stabilizing effect of the kyAB system in Bacillus thuringiensis. Secondarily, the native plasmid pBMB9741 from the same strain was resequenced and the corrected plasmid was named as pBMB7635. Based on sequence homology with the tasAB system and the lethal activity of toxin protein in Escherichia coli, a tasAB-like putative toxin-antitoxin system was identified on pBMB7635.
基于与其他毒素-抗毒素系统的序列同源性、KyB假定毒素在大肠杆菌中的致死活性以及kyAB系统在苏云金芽孢杆菌中的稳定作用，在苏云金芽孢杆菌YBT-1520菌株的新型天然质粒pBMB8240中鉴定出一种名为KyAB系统的新型假定毒素-抗毒素分离稳定系统。其次，对同一菌株的天然质粒pBMB9741进行重新测序，校正后的质粒命名为pBMB7635。基于与tasAB系统的序列同源性以及毒素蛋白在大肠杆菌中的致死活性，在pBMB7635上鉴定出一个类tasAB假定毒素-抗毒素系统。
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