精子核组蛋白H2B：与精子DNA变性及DNA染色性的相关性

Sperm nuclear histone H2B: correlation with sperm DNA denaturation and DNA stainability.

作者信息

Zini Armand, Zhang Xiaoyang, San Gabriel Maria

机构信息

Division of Urology, Department of Surgery, Royal Victoria Hospital, McGill University, Montreal, Quebec H3T 1M5, Canada.

出版信息

Asian J Androl. 2008 Nov;10(6):865-71. doi: 10.1111/j.1745-7262.2008.00415.x.

DOI:10.1111/j.1745-7262.2008.00415.x

PMID:18958350

Abstract

AIM

To examine the relationship between sperm DNA damage and sperm nuclear histone (H2B) staining.

METHODS

We evaluated sperm samples from 14 consecutive asthenoteratozoospermic infertile men and six consecutive fertile controls. Sperm nuclear histone (H2B) staining and sperm chromatin integrity (assessed by sperm chromatin structure assay and expressed using the percentage of (i) DNA fragmentation index [% DFI] and (ii) high DNA stainability [% HDS)]) were evaluated.

RESULTS

Histone H2B immunocytochemistry demonstrated two nuclear staining patterns: (i) focal punctate staining; and (ii) diffuse staining. Infertile men had a higher mean percentage of spermatozoa exhibiting diffuse H2B staining than did fertile men (7.7% +/- 4.6% vs. 1.6% +/- 1.2%, respectively, P < 0.01). We observed significant relationships between the proportion of spermatozoa with diffuse nuclear histone staining and both sperm % DFI (r = 0.63, P < 0.01) and sperm %HDS (r = 0.63, P < 0.01).

CONCLUSION

The data demonstrate that infertile men have a higher proportion of spermatozoa with diffuse histone H2B than do fertile men and suggest that sperm DNA damage might, at least in part, be due to abnormally high histone H2B levels.

摘要

目的

研究精子DNA损伤与精子核组蛋白（H2B）染色之间的关系。

方法

我们评估了14例连续性弱畸精子症不育男性和6例连续性生育力正常男性的精子样本。评估了精子核组蛋白（H2B）染色和精子染色质完整性（通过精子染色质结构分析进行评估，并以（i）DNA碎片化指数[％DFI]和（ii）高DNA染色性[％HDS]的百分比表示）。

结果

组蛋白H2B免疫细胞化学显示出两种核染色模式：（i）局灶点状染色；和（ii）弥漫性染色。不育男性中表现出弥漫性H2B染色的精子平均百分比高于生育力正常男性（分别为7.7％±4.6％和1.6％±1.2％，P<0.01）。我们观察到具有弥漫性核组蛋白染色的精子比例与精子％DFI（r = 0.63，P<0.01）和精子％HDS（r = 0.63，P<0.01）之间存在显著关系。