Patonay G, Casay G A, Lipowska M, Strekowski L
Department of Chemistry, Georgia State University, Atlanta, GA 30303, U.S.A.
Talanta. 1993 Jun;40(6):935-42. doi: 10.1016/0039-9140(93)80055-v.
Synthetic red and near-infrared absorbing dyes may be used as probe molecules in a large number of applications. Dyes exhibiting spectral changes with hydrogen ion concentration are useful as pH probes. Those dyes which have their absorption and fluorescence maxima in the long wavelength region of the visible spectral region are specially valuable because of decreased interference and semiconductor laser applications. In this paper we have evaluated an aminodienone dyes 1 which demostrates pH dependent absorption and fluorescence spectra as well as solvent polarity dependence. In organic solvents the long wavelength absorption band of the dye is in the reduced interference region. The absorption maximum is at 535 nm in neutral or alkaline solutions in methanol. The absorption spectra undergo a strong bathochromic shift in the presence of acids (lambda(max) = 709 nm) with a concomitant change in the fluorescence spectra. This pH sensitive dye was found to be specially especially useful for organic solvents. The analytical utility of this and similar near-infrared absorbing dyes is discussed.
合成的红色和近红外吸收染料可在大量应用中用作探针分子。随氢离子浓度呈现光谱变化的染料可用作pH探针。那些在可见光谱区域的长波长区域具有吸收和荧光最大值的染料特别有价值，因为干扰减少且适用于半导体激光应用。在本文中，我们评估了一种氨基二烯酮染料1，它表现出pH依赖性吸收和荧光光谱以及溶剂极性依赖性。在有机溶剂中，该染料的长波长吸收带处于干扰减少的区域。在甲醇中的中性或碱性溶液中，最大吸收波长为535nm。在酸存在下，吸收光谱会发生强烈的红移（λ(max)=709nm），同时荧光光谱也会发生变化。发现这种pH敏感染料对有机溶剂特别有用。本文讨论了这种以及类似的近红外吸收染料的分析用途。
