核酸损伤的傅里叶变换红外光谱研究。

FTIR spectroscopy studies of nucleic acid damage.

作者信息

Dovbeshko G I, Gridina N Y, Kruglova E B, Pashchuk O P

机构信息

Institute of Physics, National Academy of Sciences of Ukraine, 46 Prospekt Nauki, Kiev 03039, Ukraine.

出版信息

Talanta. 2000 Oct 2;53(1):233-46. doi: 10.1016/s0039-9140(00)00462-8.

DOI:10.1016/s0039-9140(00)00462-8

PMID:18968108

Abstract

Fourier transform infrared (FTIR) spectra of RNA isolated from tumour brain (glioma) and DNA isolated from low-dose gamma-irradiated epididymis cells of rats from the Chernobyl accident zone have been investigated in the 700-4000 cm(-1) region. The total absorbed radiation doses were equal to or less than 57 cGy. Observed changes in the FTIR spectra represent the damage in the primary, secondary and tertiary structure of nucleic acid, which seem to be connected with modification of bases and sugars, and redistribution of the H-bond network. FTIR data for tumour and gamma-irradiated nucleic acid show some similar features. A great amount of statistical data and good mathematical approaches are needed for the use of these data as diagnostic criteria.

摘要

对从切尔诺贝利事故区域大鼠的肿瘤脑（胶质瘤）中分离出的RNA以及从低剂量γ射线辐照的附睾细胞中分离出的DNA的傅里叶变换红外（FTIR）光谱，在700 - 4000 cm⁻¹区域进行了研究。总吸收辐射剂量等于或小于57 cGy。FTIR光谱中观察到的变化代表了核酸一级、二级和三级结构的损伤，这似乎与碱基和糖的修饰以及氢键网络的重新分布有关。肿瘤和γ射线辐照核酸的FTIR数据显示出一些相似特征。要将这些数据用作诊断标准，需要大量的统计数据和良好的数学方法。