使用普鲁士蓝膜修饰电极流动注射安培法检测抗坏血酸

Flow injection amperometric detection of ascorbic acid using a Prussian Blue film-modified electrode.

作者信息

Castro S S, Balbo V R, Barbeira P J, Stradiotto N R

机构信息

Departamento de Quimica Analitica, Instituto de Quimica, UNESP, 14800-900, Araraquara, SP, Brazil.

出版信息

Talanta. 2001 Aug 30;55(2):249-54. doi: 10.1016/s0039-9140(01)00407-6.

DOI:10.1016/s0039-9140(01)00407-6

PMID:18968367

Abstract

The PB film-modified electrode was used as an amperometric detector for flow injection analysis of ascorbic acid. The modified electrode detector showed good sensitivity, stability and reproducibility. The calibration curve for ascorbic acid was linear over the concentration range from 5.0x10(-6) to 1.0x10(-3) mol l(-1) with a slope of 19.9 mA mol(-1) per litre and a correlation coefficient of 0.999. The detection limit of this method was 2.49x10(-6) mol l(-1). The relative standard deviation of six replicate injections of 2.5x10(-4) mol l(-1) ascorbic acid was 2.5%. The results obtained for ascorbic acid determination in pharmaceutical products are in good agreement with those obtained by using the procedure involving the reaction between triiodide and ascorbic acid.

摘要

将PB膜修饰电极用作安培检测器，用于流动注射分析抗坏血酸。修饰电极检测器显示出良好的灵敏度、稳定性和重现性。抗坏血酸的校准曲线在5.0×10⁻⁶至1.0×10⁻³ mol l⁻¹的浓度范围内呈线性，斜率为每升19.9 mA mol⁻¹，相关系数为0.999。该方法的检测限为2.49×10⁻⁶ mol l⁻¹。2.5×10⁻⁴ mol l⁻¹抗坏血酸六次重复进样的相对标准偏差为2.5%。药品中抗坏血酸测定的结果与采用涉及碘化物与抗坏血酸反应的方法所获得的结果高度一致。