通过激光诱导荧光检测的毛细管电泳法定量测定人血浆和烟草叶片中的谷胱甘肽和二硫化谷胱甘肽。

Quantification of glutathione and glutathione disulfide in human plasma and tobacco leaves by capillary electrophoresis with laser-induced fluorescence detection.

作者信息

Zhang Jiyou, Hu Zhide, Chen Xingguo

机构信息

Department of Chemistry, Lanzhou University, Lanzhou 730000, P.R. China.

出版信息

Talanta. 2005 Feb 28;65(4):986-90. doi: 10.1016/j.talanta.2004.08.030.

DOI:10.1016/j.talanta.2004.08.030

PMID:18969899

Abstract

A new capillary electrophoresis (CE) method with laser-induced fluorescence (LIF) detection was developed for the rapid separation and sensitive detection of glutathione (GSH) and glutathione disulfide (GSSH) after derivatization by 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol (NBD-Cl). The derivatization and separation conditions were investigated in detail and the optimums were obtained. Under the optimum experiment conditions, linear relationships between the peak height and concentrations of the analytes in normal and second-derivative electrophoregrams were obtained (0.22-45.00muM). The detection limits for glutathione and glutathione disulfide in normal and second-derivative electrophoregrams were 0.046 and 0.012muM and 0.046 and 0.014muM, respectively. The method was applied to the analysis of glutathione and glutathione disulfide in human plasma and tobacco leaves with satisfactory results.

摘要

建立了一种新的毛细管电泳（CE）方法，该方法采用激光诱导荧光（LIF）检测，用于在4-氯-7-硝基苯并-2-恶唑-1,3-二唑（NBD-Cl）衍生化后快速分离和灵敏检测谷胱甘肽（GSH）和谷胱甘肽二硫化物（GSSH）。详细研究了衍生化和分离条件并获得了最佳条件。在最佳实验条件下，在正常和二阶导数电泳图中获得了峰高与分析物浓度之间的线性关系（0.22 - 45.00μM）。正常和二阶导数电泳图中谷胱甘肽和谷胱甘肽二硫化物的检测限分别为0.046和0.012μM以及0.046和0.014μM。该方法应用于人体血浆和烟草叶片中谷胱甘肽和谷胱甘肽二硫化物的分析，结果令人满意。