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[犬猫粪便中产肠毒素性产气荚膜梭菌菌株的出现情况]

[The occurrence of enterotoxigenic Clostridium perfringens strains in the feces of dogs and cats].

作者信息

Werdeling F, Amtsberg G, Tewes S

机构信息

Institut für Mikrobiologie und Tierseuchen, Tierärztlichen Hochschule Hannover.

出版信息

Berl Munch Tierarztl Wochenschr. 1991 Jul 1;104(7):228-33.

PMID:1898321
Abstract

A total of 147 faecal specimen of dogs and cats was examined with cultural method for the occurrence of Cl. perfringens and its enterotoxin by using a reversed passive latex agglutination test (Pet-RPLA). Cl. perfringens could be detected in 77.9% of the samples of dogs (n = 68) and 65.6% of cats (n = 32) with diarrhoea in germ counts of 10(4)-10(10) cfu/g faeces. In the group of non diarrhoeic dogs (n = 39) and cats (n = 8) Cl. perfringens was found in 53.9% and 50% of the samples, the germ counts revealed 10(4)-10(8) cfu/g faeces. The enterotoxin of Cl. perfringens was detected in 48.5% of the samples of dogs and 28.1% of cats with diarrhoea. On the other hand this toxin could not be detected in any faecal specimen of non diarrhoeic animals. The in vitro detection of Cl. perfringens enterotoxin was successful at 65% of 20 strains, that had been isolated from faecal samples with enterotoxin. Also 2 Cl. perfringens strains isolated from faeces of non diarrhoeic dogs proved to be enterotoxigenic. The present test results given reason to believe that enterotoxigenic Cl. perfringens strains are involved in the complex of enteric disease of dogs and cats.

摘要

采用反向被动乳胶凝集试验(Pet-RPLA),通过培养方法对147份犬猫粪便样本进行产气荚膜梭菌及其肠毒素检测。在腹泻犬样本(n = 68)中,77.9%检测到产气荚膜梭菌,菌数为10⁴-10¹⁰ cfu/g粪便;腹泻猫样本(n = 32)中,65.6%检测到产气荚膜梭菌,菌数为10⁴-10¹⁰ cfu/g粪便。在非腹泻犬组(n = 39)和猫组(n = 8)中,分别有53.9%和50%的样本检测到产气荚膜梭菌,菌数为10⁴-10⁸ cfu/g粪便。产气荚膜梭菌肠毒素在腹泻犬样本的48.5%和腹泻猫样本的28.1%中被检测到。另一方面,在非腹泻动物的粪便样本中未检测到该毒素。从含肠毒素粪便样本中分离出的20株菌株,65%的菌株成功进行了产气荚膜梭菌肠毒素的体外检测。从非腹泻犬粪便中分离出的2株产气荚膜梭菌菌株也被证明具有产肠毒素能力。目前的检测结果有理由相信,产肠毒素的产气荚膜梭菌菌株与犬猫肠道疾病复合体有关。

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