Suppr超能文献

朊病毒在体外的传播：我们做到了吗？

Prion propagation in vitro: are we there yet?

作者信息

Ryou Chongsuk, Mays Charles E

机构信息

Sanders Brown Center on Aging and Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY 40536, USA.

出版信息

Int J Med Sci. 2008;5(6):347-53. doi: 10.7150/ijms.5.347. Epub 2008 Nov 11.

DOI:10.7150/ijms.5.347
PMID:19015743
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2583336/
Abstract

Prion diseases are caused by proteinaceous pathogens termed prions. Although the details of the mechanism of prion propagation are not fully understood, conformational conversion of cellular prion protein (PrP(C)) to misfolded, disease-associated scrapie prion protein (PrP(Sc)) is considered the essential biochemical event for prion replication. Currently, studying prion replication in vitro is difficult due to the lack of a system which fully recapitulates the in vivo phenomenon. Over the last 15 years, a number of in vitro systems supporting PrP(C) conversion, PrP(Sc) amplification, or amyloid fibril formation have been established. In this review, we describe the evolving methodology of in vitro prion propagation assays and discuss their ability in reflecting prion propagation in vivo.

摘要

朊病毒疾病由被称为朊病毒的蛋白质病原体引起。尽管朊病毒传播机制的细节尚未完全明了，但细胞朊蛋白（PrP(C)）向错误折叠的、与疾病相关的瘙痒病朊病毒蛋白（PrP(Sc)）的构象转变被认为是朊病毒复制的关键生化事件。目前，由于缺乏能完全重现体内现象的系统，体外研究朊病毒复制很困难。在过去15年里，已建立了许多支持PrP(C)转化、PrP(Sc)扩增或淀粉样纤维形成的体外系统。在这篇综述中，我们描述了体外朊病毒传播测定方法的演变，并讨论了它们反映体内朊病毒传播的能力。

相似文献

1
Prion propagation in vitro: are we there yet?
Int J Med Sci. 2008;5(6):347-53. doi: 10.7150/ijms.5.347. Epub 2008 Nov 11.
2
Generation of genuine prion infectivity by serial PMCA.
Vet Microbiol. 2007 Aug 31;123(4):346-57. doi: 10.1016/j.vetmic.2007.04.004. Epub 2007 Apr 7.
3
Breakage of PrP aggregates is essential for efficient autocatalytic propagation of misfolded prion protein.
Biochem Biophys Res Commun. 2005 Jan 14;326(2):339-43. doi: 10.1016/j.bbrc.2004.11.039.
4
Efficient in vitro amplification of a mouse-adapted scrapie prion protein.
Neurosci Lett. 2007 Feb 21;413(3):270-3. doi: 10.1016/j.neulet.2006.11.056. Epub 2006 Dec 14.
5
In vitro amplification of scrapie and chronic wasting disease PrP(res) using baculovirus-expressed recombinant PrP as substrate.
Prion. 2014;8(6):393-403. doi: 10.4161/19336896.2014.983753.
6
Prion disease is accelerated in mice lacking stress-induced heat shock protein 70 (HSP70).
J Biol Chem. 2019 Sep 13;294(37):13619-13628. doi: 10.1074/jbc.RA118.006186. Epub 2019 Jul 18.
7
Emergence of two prion subtypes in ovine PrP transgenic mice infected with human MM2-cortical Creutzfeldt-Jakob disease prions.
Acta Neuropathol Commun. 2016 Feb 5;4:10. doi: 10.1186/s40478-016-0284-9.
8
Cell-free formation of misfolded prion protein with authentic prion infectivity.
Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15818-23. doi: 10.1073/pnas.0605608103. Epub 2006 Oct 9.
9
Crossing the species barrier by PrP(Sc) replication in vitro generates unique infectious prions.
Cell. 2008 Sep 5;134(5):757-68. doi: 10.1016/j.cell.2008.07.030.
10
Prion Protein Devoid of the Octapeptide Repeat Region Delays Bovine Spongiform Encephalopathy Pathogenesis in Mice.
J Virol. 2017 Dec 14;92(1). doi: 10.1128/JVI.01368-17. Print 2018 Jan 1.

引用本文的文献

1
Synthesis and anti-prion aggregation activity of acylthiosemicarbazide analogues.
J Enzyme Inhib Med Chem. 2023 Dec;38(1):2191164. doi: 10.1080/14756366.2023.2191164.
2
In vitro Modeling of Prion Strain Tropism.
Viruses. 2019 Mar 9;11(3):236. doi: 10.3390/v11030236.
3
Design, implementation, and interpretation of amplification studies for prion detection.
Prion. 2018 Mar 4;12(2):73-82. doi: 10.1080/19336896.2018.1443000. Epub 2018 Mar 9.
4
Parameters that affect macromolecular self-assembly of prion protein.
Protein J. 2014 Jun;33(3):243-52. doi: 10.1007/s10930-014-9556-z.
5
Slow spontaneous α-to-β structural conversion in a non-denaturing neutral condition reveals the intrinsically disordered property of the disulfide-reduced recombinant mouse prion protein.
Prion. 2012 Nov-Dec;6(5):489-97. doi: 10.4161/pri.22217. Epub 2012 Sep 17.
6
Is tau ready for admission to the prion club?
Prion. 2012 Jul 1;6(3):223-33. doi: 10.4161/pri.19912.
7
In vitro amplification of misfolded prion protein using lysate of cultured cells.
PLoS One. 2011 Mar 28;6(3):e18047. doi: 10.1371/journal.pone.0018047.
8
Enhancement of protein misfolding cyclic amplification by using concentrated cellular prion protein source.
Biochem Biophys Res Commun. 2009 Oct 16;388(2):306-10. doi: 10.1016/j.bbrc.2009.07.163. Epub 2009 Aug 5.

本文引用的文献

1
Simplified ultrasensitive prion detection by recombinant PrP conversion with shaking.
Nat Methods. 2008 Mar;5(3):211-2. doi: 10.1038/nmeth0308-211.
2
Prions and prion diseases: fundamentals and mechanistic details.
J Microbiol Biotechnol. 2007 Jul;17(7):1059-70.
3
Selective incorporation of polyanionic molecules into hamster prions.
J Biol Chem. 2007 Dec 14;282(50):36341-53. doi: 10.1074/jbc.M704447200. Epub 2007 Oct 16.
4
Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein.
Nat Methods. 2007 Aug;4(8):645-50. doi: 10.1038/nmeth1066. Epub 2007 Jul 22.
5
Formation of native prions from minimal components in vitro.
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9741-6. doi: 10.1073/pnas.0702662104. Epub 2007 May 29.
6
Synergistic and strain-specific effects of bovine spongiform encephalopathy and scrapie prions in the cell-free conversion of recombinant prion protein.
J Gen Virol. 2006 Dec;87(Pt 12):3753-3761. doi: 10.1099/vir.0.81590-0.
7
Protein misfolding cyclic amplification for diagnosis and prion propagation studies.
Methods Enzymol. 2006;412:3-21. doi: 10.1016/S0076-6879(06)12001-7.
8
Copper (II) ions potently inhibit purified PrPres amplification.
J Neurochem. 2006 Mar;96(5):1409-15. doi: 10.1111/j.1471-4159.2006.03650.x. Epub 2006 Jan 17.
9
The most infectious prion protein particles.
Nature. 2005 Sep 8;437(7056):257-61. doi: 10.1038/nature03989.
10
Protease-resistant prion protein amplification reconstituted with partially purified substrates and synthetic polyanions.
J Biol Chem. 2005 Jul 22;280(29):26873-9. doi: 10.1074/jbc.M503973200. Epub 2005 May 24.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验