Souza Marcella S, Aguieiras Erika C G, da Silva Mônica A P, Langone Marta A P
Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Appl Biochem Biotechnol. 2009 May;154(1-3):74-88. doi: 10.1007/s12010-008-8444-4. Epub 2008 Dec 9.
The objective of this work was to study the synthesis of ethyl esters via esterification of soybean oil deodorizer distillate with ethanol, using solid acid catalysts and commercial immobilized lipases, in a solvent-free system. Three commercially immobilized lipases were used, namely, Lipozyme RM-IM, Lipozyme TL-IM, and Novozym 435, all from Novozymes. We aimed for optimum reaction parameters: temperature, enzyme concentration, initial amount of ethanol, and its feeding technique to the reactor (stepwise ethanolysis). Reaction was faster with Novozym 435. The highest conversion (83.5%) was obtained after 90 min using 3 wt.% of Novozym 435 and two-stage stepwise addition of ethanol at 50 degrees C. Four catalysts were also tested: zeolite CBV-780, SAPO-34, niobia, and niobic acid. The highest conversion (30%) was obtained at 100 degrees C, with 3 wt.% of CBV-780 after 2.5 h. The effects of zeolite CBV 780 concentration were studied, resulting in a conversion of 49% using 9 wt.% of catalyst.
本工作的目的是在无溶剂体系中，研究使用固体酸催化剂和商业固定化脂肪酶，通过大豆油脱臭馏出物与乙醇的酯化反应合成乙酯。使用了三种商业固定化脂肪酶，即均来自诺维信公司的Lipozyme RM-IM、Lipozyme TL-IM和诺维信435。我们旨在确定最佳反应参数：温度、酶浓度、乙醇初始量及其加入反应器的技术（分步醇解）。使用诺维信435时反应更快。在50℃下，使用3 wt.%的诺维信435并分两阶段逐步添加乙醇，90分钟后获得了最高转化率（83.5%）。还测试了四种催化剂：沸石CBV-780、SAPO-34、氧化铌和铌酸。在100℃下，使用3 wt.%的CBV-780，2.5小时后获得了最高转化率（30%）。研究了沸石CBV 780浓度的影响，使用9 wt.%的催化剂时转化率为49%。
