Cai Meng-meng, Chua Hong, Wong Ai-ling Phoeby, Yu Hoi-fu Peter, Sin Ngai Shirley, Ren Jie, He Dan, Zhao Qing-liang
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Huan Jing Ke Xue. 2008 Sep;29(9):2643-8.
To reduce the production cost of polyhydroxyalkanoates (PHAs), the process feasibility and physicochemical properties of PHAs synthesized by Alcaligenes latus, Staphylococcus epidermidis and activated sludge from malt waste, soy waste, confectionary waste, ice cream waste, milk waste, sesame oil and vinegar waste were analyzed. Results showed that through two-stage fed-batch fermentation, the maximum yield of PHAs accumulated by the three kinds of microorganisms from malt waste was 70.1%, 16.0% and 43.3%, separately. A. latus adapted itself to the food wastes in PHAs synthesis and new cell growth quickly. A. latus had higher PHAs yield and productivity under nitrogen limited condition. Micro-aerobically, S. epidermidis separated from sesame oil could produce polyhydroxybutyrate (PHB) with molecular weight of over 1 x 10(6). From soy waste, activated sludge accumulated polyhydroxybutyrate-co-hydroxyvaluate (PHBV) copolymer which had hydroxyvaleryl content (HV%) of 21%. Most food wastes are suitable for synthesizing PHAs with different physicochemical properties. The composition and properties of PHAs are influenced by the character of microorganism, the selection of substrates and optimization of ferment conditions.
为降低聚羟基脂肪酸酯(PHA)的生产成本,对产碱杆菌、表皮葡萄球菌以及麦芽废料、大豆废料、糖果废料、冰淇淋废料、牛奶废料、芝麻油和醋废料的活性污泥合成的PHA的工艺可行性和理化性质进行了分析。结果表明,通过两阶段补料分批发酵,三种微生物从麦芽废料中积累的PHA的最大产量分别为70.1%、16.0%和43.3%。产碱杆菌在PHA合成中能快速适应食品废料并进行新细胞生长。在氮限制条件下,产碱杆菌具有较高的PHA产量和生产率。在微需氧条件下,从芝麻油中分离出的表皮葡萄球菌可产生分子量超过1×10⁶的聚羟基丁酸酯(PHB)。活性污泥从大豆废料中积累了羟基戊酸含量(HV%)为21%的聚羟基丁酸酯-共-羟基戊酸酯(PHBV)共聚物。大多数食品废料适合合成具有不同理化性质的PHA。PHA的组成和性质受微生物特性、底物选择和发酵条件优化的影响。