采用高速逆流色谱法从太子参（Pseudostellaria heterophylla (Miq.) Pax）中分离纯化假太子参环肽B（环状肽） 。
Isolation and purification of Pseudostellarin B (cyclic peptide) from Pseudostellaria heterophylla (Miq.) Pax by high-speed counter-current chromatography.
作者信息
Han Chao, Chen Junhui, Liu Jie, Lee Frank Sen-Chun, Wang Xiaoru
机构信息
Department of Chemistry and the Key Laboratory of Analytical Science of the MOE, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
出版信息
Talanta. 2007 Feb 15;71(2):801-5. doi: 10.1016/j.talanta.2006.05.016. Epub 2006 Jun 21.
Pseudostellarin B (cyclic peptide) was isolated and purified from the herbs of Pseudostellaria heterophylla (Miq.) Pax for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system consisting of n-butanol-ethyl acetate-water (0.6:4.4:5, v/v). The technique can isolate mg levels of the target compound per run with purity better than 96%. The chemical structure of the compound has been positively confirmed by electrospray ionization time of flight (TOF) MS, (1)H-NMR, (13)C-NMR and (1)H-(13)C-COSY analyses.
首次采用高速逆流色谱法（HSCCC），以正丁醇 - 乙酸乙酯 - 水（0.6:4.4:5，v/v）组成的两相溶剂系统，从太子参（Pseudostellaria heterophylla (Miq.) Pax）的草药中分离并纯化出假太子参环肽B（环状肽）。该技术每次运行可分离出毫克级的目标化合物，纯度高于96%。该化合物的化学结构已通过电喷雾电离飞行时间（TOF）质谱、（1）H - NMR、（13）C - NMR和（1）H - （13）C - COSY分析得到确证。
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