白色念珠菌对内皮细胞的刺激作用。
Endothelial cell stimulation by Candida albicans.
作者信息
Phan Quynh T, Filler Scott G
机构信息
Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, California, USA.
出版信息
Methods Mol Biol. 2009;470:313-26. doi: 10.1007/978-1-59745-204-5_22.
The opportunistic fungal pathogen Candida albicans enters the bloodstream and causes hematogenously disseminated infection in hospitalized patients. During the initiation of a hematogenously disseminated infection, endothelial cells are one of the first host cells to come in contact with C. albicans. Endothelial cells can significantly influence the local host response to C. albicans by expressing leukocyte adhesion molecules and proinflammatory cytokines. Thus, it is of interest to investigate the response of endothelial cells to C. albicans in vitro. We describe the use of real-time PCR and enzyme immunoassays to measure the effects of C. albicans on the endothelial cell production of E-selectin and tumor necrosis factor alpha in vitro.
机会性真菌病原体白色念珠菌进入血流并在住院患者中引起血行播散性感染。在血行播散性感染开始时，内皮细胞是最早与白色念珠菌接触的宿主细胞之一。内皮细胞可通过表达白细胞粘附分子和促炎细胞因子，显著影响局部宿主对白色念珠菌的反应。因此，研究内皮细胞在体外对白色念珠菌的反应具有重要意义。我们描述了使用实时PCR和酶免疫测定法来测量白色念珠菌在体外对内皮细胞产生E-选择素和肿瘤坏死因子α的影响。
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