精液中细菌种类的存在与精子质量
The presence of bacteria species in semen and sperm quality.
作者信息
Moretti Elena, Capitani Serena, Figura Natale, Pammolli Andrea, Federico Maria Grazia, Giannerini Valentina, Collodel Giulia
机构信息
Department of Biomedical Sciences, Biology Section, Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Policlinico Le Scotte, Siena, Italy.
出版信息
J Assist Reprod Genet. 2009 Jan;26(1):47-56. doi: 10.1007/s10815-008-9283-5. Epub 2008 Dec 17.
PURPOSE
To verify the prevalence of semen bacterial contamination and whether the contamination could decrease sperm quality.
METHODS
Spermiogram, semen culture, and sperm transmission electron microscopy (TEM) analysis were performed. TEM data were elaborated using a mathematical formula that calculates a fertility index (FI)--able to define patients as fertile or infertile--and the percentage of sperm apoptosis, immaturity and necrosis. We aligned the amino acid sequence of beta-tubulin with protein of the most frequent species isolated from semen.
RESULTS
Patients were divided according to the contaminating species; in each group, we observed fertile individuals, in whom the semen quality was similar to that of controls and infertile men whose sperm quality was significantly decreased, in terms of motility, FI, apoptosis and necrosis. Partial homology between beta-tubulin and bacterial proteins was observed.
CONCLUSION
Sperm bacterial contamination is quite frequent and could contribute to the deterioration of the sperm quality of infertile men.
目的
验证精液细菌污染的发生率以及该污染是否会降低精子质量。
方法
进行精液分析、精液培养和精子透射电子显微镜（TEM）分析。使用一个计算生育指数（FI）的数学公式对TEM数据进行阐述，该指数能够将患者定义为有生育能力或无生育能力，并计算精子凋亡、不成熟和坏死的百分比。我们将β-微管蛋白的氨基酸序列与从精液中分离出的最常见菌种的蛋白质进行比对。
结果
根据污染菌种对患者进行分组；在每组中，我们观察到有生育能力的个体，其精液质量与对照组相似，以及无生育能力的男性，其精子质量在活力、FI、凋亡和坏死方面显著下降。观察到β-微管蛋白与细菌蛋白之间存在部分同源性。
结论
精子细菌污染相当常见，可能导致无生育能力男性的精子质量恶化。
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