[成人乳糜泻与上皮内淋巴细胞。诊断的新选择？]
[Adult celiac disease and intraepithelial lymphocytes. New options for diagnosis?].
作者信息
Olivencia Palomar Pilar, Cano Ruiz Ana, Martín Scapa M Angeles, León Prieto Francisco, Roy Ariño Garbiñe, Redondo Verge Clara
机构信息
Servicio de Aparato Digestivo, Hospital Universitario Ramón y Cajal, Madrid, España.
出版信息
Gastroenterol Hepatol. 2008 Nov;31(9):555-9. doi: 10.1157/13128293.
BACKGROUND
Permanent changes have been found in intraepithelial lymphocyte (IEL) subsets in patients with celiac disease.
OBJECTIVE
The main aim of this study was to demonstrate the utility of determining CD3()/CD7(+) and T cell receptor (TCR) gamma-delta IEL subsets by flow cytometry as a diagnostic marker of adult celiac disease.
PATIENTS AND METHODS
We performed a prospective study in a sample of 128 adult patients (70 with celiac disease and 58 controls). In all patients, distal duodenal biopsy was performed and IEL subsets were determined by flow cytometry.
RESULTS
Patients with celiac disease showed an increase in gamma-delta IEL subsets and a decrease in CD3(-)/CD7(+) IEL subsets in comparison with the control group, independently of diet.
CONCLUSIONS
The results indicate that IEL subset determination by flow cytometry could be useful to confirm diagnosis of celiac disease. IEL subsets should be investigated in diseases other than celiac disease, as well as in patients with potential or latent celiac disease.
背景
乳糜泻患者的上皮内淋巴细胞（IEL）亚群存在永久性变化。
目的
本研究的主要目的是证明通过流式细胞术检测CD3()/CD7(+)和T细胞受体（TCR）γ-δ IEL亚群作为成人乳糜泻诊断标志物的实用性。
患者与方法
我们对128例成年患者（70例乳糜泻患者和58例对照）进行了一项前瞻性研究。所有患者均进行了十二指肠远端活检，并通过流式细胞术确定IEL亚群。
结果
与对照组相比，乳糜泻患者的γ-δ IEL亚群增加，CD3(-)/CD7(+) IEL亚群减少，且与饮食无关。
结论
结果表明，通过流式细胞术检测IEL亚群有助于确诊乳糜泻。除乳糜泻外，还应在其他疾病以及潜在或隐匿性乳糜泻患者中研究IEL亚群。
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