Pappu H R, Druffel K L
Department of Plant Pathology, Washington State University, PO Box 646430, Pullman, WA 99164-6430, USA.
J Virol Methods. 2009 Apr;157(1):102-4. doi: 10.1016/j.jviromet.2008.11.014. Epub 2009 Jan 22.
The genus Caulimovirus consists of several distinct virus species with a double-stranded DNA genome that infect diverse plant species. A comparative analysis of the sequences of known Caulimovirus species revealed two regions that are conserved in all Caulimovirus species with the exception of Strawberry vein banding virus. Degenerate primers based on these two regions were designed and tested in a polymerase chain reaction-based assay for broad spectrum detection of members of this genus. Cauliflower mosaic virus, Figwort mosaic virus and three distinct caulimoviruses associated with dahlia (Dahlia variabilis) were used to show the utility of this test in detecting diverse caulimoviruses. The primer pair gave an amplicon of expected size (840bp). Amplicons from each virus were cloned and sequenced to verify their identity. The primer pair and the PCR assay provide approach for the broad spectrum detection of several members of the genus Caulimovirus.
花椰菜花叶病毒属由几种不同的病毒物种组成，它们具有双链DNA基因组，可感染多种植物物种。对已知花椰菜花叶病毒属物种的序列进行比较分析后发现，除草莓脉带病毒外，所有花椰菜花叶病毒属物种中都有两个区域是保守的。基于这两个区域设计了简并引物，并在基于聚合酶链反应的检测中进行测试，以用于广谱检测该属的成员。使用花椰菜花叶病毒、玄参花叶病毒以及三种与大丽花（大丽花变种）相关的不同花椰菜花叶病毒，来展示该检测方法在检测不同花椰菜花叶病毒方面的实用性。引物对产生了预期大小（840bp）的扩增子。对每种病毒的扩增子进行克隆和测序，以验证其身份。该引物对和聚合酶链反应检测方法为广谱检测花椰菜花叶病毒属的几个成员提供了途径。
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