干扰素-α与原发性干燥综合征之间的关联。

Association between IFN-alpha and primary Sjogren's syndrome.

作者信息

Zheng Lingyan, Zhang Zhiyuan, Yu Chuangqi, Tu Liwei, Zhong Laiping, Yang Chi

机构信息

Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Jan;107(1):e12-8. doi: 10.1016/j.tripleo.2008.09.015.

DOI:10.1016/j.tripleo.2008.09.015

PMID:19101478

Abstract

OBJECTIVE

To determine the level of IFN-alpha in labial salivary glands, plasma, and peripheral blood cells from patients with primary Sjogren's syndrome (pSS).

METHODS

Labial salivary gland biopsy specimens, plasma, and peripheral blood cells from patients with pSS were investigated. The IFN-alpha-positive cells, measurable IFN-alpha level, and IFN-alpha gene mRNA level were determined by using immunohistochemistry, ELISA, and real-time PCR, respectively. Statistical analysis was performed by Student t test or Fisher's exact test.

RESULTS

About 60% of patients (22/37) with pSS had significantly higher scores of IFN-alpha-positive cells in labial gland biopsy and most IFN-alpha-positive cells were localized predominantly in the lymphocytes and ductal epithelial cells. But in 3 of the control samples (3/24), the IFN-alpha-positive cells existed only in the ductal epithelial cells with lower scores. Forty-three percents of the patients with pSS were found with detectable IFN-alpha concentration in plasma (> or = 12.5 pg/mL), and their concentration was higher than that of control group. Furthermore, the IFN-alpha mRNA levels in peripheral blood cells were up-regulated in the patients with pSS.

CONCLUSION

No matter in labial salivary glands or hematoplasma, or peripheral blood cells, IFN-alpha expression levels are up-regulated in patients with primary Sjogren's syndrome.

摘要

目的

测定原发性干燥综合征（pSS）患者唇腺、血浆及外周血细胞中α干扰素（IFN-α）的水平。

方法

对pSS患者的唇腺活检标本、血浆及外周血细胞进行研究。分别采用免疫组化、酶联免疫吸附测定（ELISA）及实时聚合酶链反应（PCR）来测定IFN-α阳性细胞、可检测的IFN-α水平及IFN-α基因信使核糖核酸（mRNA）水平。采用学生t检验或费舍尔精确检验进行统计学分析。

结果

约60%的pSS患者（22/37）唇腺活检中IFN-α阳性细胞评分显著更高，且大多数IFN-α阳性细胞主要定位于淋巴细胞和导管上皮细胞。但在3份对照样本（3/24）中，IFN-α阳性细胞仅存在于导管上皮细胞中，且评分较低。发现43%的pSS患者血浆中可检测到IFN-α浓度（≥12.5 pg/mL），且其浓度高于对照组。此外，pSS患者外周血细胞中的IFN-α mRNA水平上调。