Hua Susan Z, Pennell Thomas
Bio-MEMS and Biomaterials Laboratory, Department of Mechanical & Aerospace Engineering, SUNY-Buffalo, Buffalo, NY 14260, USA.
Lab Chip. 2009 Jan 21;9(2):251-6. doi: 10.1039/b806003g. Epub 2008 Oct 23.
We report a microfluidic chip that is capable of measuring volume changes in single cells in real-time. Single eukaryotic cells were immobilized in the sensing area and changes in volume in response to hypotonic challenges and drugs were measured using the electrical impedance method. Experiments on MDCK cells showed that the maximum swelling and the time course of swelling vary between individual cells following hypotonic stimulation. The microfluidic chip allows, rapid and convenient change of solutions, enabling detailed studies of various drugs and chemicals that may play important role in cell physiology at the single cell level.
我们报道了一种能够实时测量单细胞体积变化的微流控芯片。单个真核细胞被固定在传感区域,并使用电阻抗方法测量其对低渗刺激和药物的体积变化。对MDCK细胞的实验表明,低渗刺激后单个细胞的最大肿胀程度和肿胀时间进程各不相同。该微流控芯片允许快速方便地更换溶液,从而能够在单细胞水平上对可能在细胞生理学中起重要作用的各种药物和化学物质进行详细研究。