突尼斯宫颈癌女性针对L1、E6、E7人乳头瘤病毒16型和人乳头瘤病毒18型抗原的抗体反应。

Antibody response for L1, E6, E7 HPV 16, and HPV 18 antigens in Tunisian women with cervical cancer.

作者信息

Achour M, Zeghal D, Kochbati L, Kahla S, Zouari F, Maâlej M, Oueslati R

机构信息

Unit of Immuno-Microbiology Environmental and Cancerogenesis (IMEC), Faculty of Sciences, 7021 Zarzouna Bizerta, Tunisia.

出版信息

J Immunoassay Immunochem. 2009;30(1):82-96. doi: 10.1080/15321810802569543.

DOI:10.1080/15321810802569543

PMID:19117204

Abstract

Results obtained in the present work indicated that the Luminex assay is more sensitive than ELISA. The reactivity to the early antigens E6 and E7 was 37% versus 42% for HPV 16 and 21% versus 20% for HPV 18 among cervical cancer cases using ELISA. However, these ratios were 44% and 61%, respectively, for E6 and E7 HPV 16 versus 28% and 21% for E6 and E7 HPV 18 when using the Luminex technique. Data also indicated that HPV 16 and HPV 18 showed distinct profiles for the different antigens tested. Finally, the differences in antibody responses between cervical cancer cases and benign cases toward the different antigens were significant.

摘要

本研究获得的结果表明，Luminex检测法比ELISA更灵敏。在宫颈癌病例中，使用ELISA检测时，针对早期抗原E6和E7的反应性，HPV 16分别为37%和42%，HPV 18分别为21%和20%。然而，使用Luminex技术检测时，HPV 16的E6和E7的这些比率分别为44%和61%，HPV 18的E6和E7分别为28%和21%。数据还表明，HPV 16和HPV 18针对不同检测抗原呈现出不同的谱型。最后，宫颈癌病例和良性病例针对不同抗原的抗体反应差异显著。