Padilla F C, Rincón A M, Bou-Rached L
Universidad Central de Venezuela, Facultad de Farmacia, Unidad de Análisis de Alimentos, Caracas, Venezuela.
Arch Latinoam Nutr. 2008 Sep;58(3):303-8.
Foods from plant origin not only provide human diet with certain antioxidant vitamins (C, E and beta-carotene), but also a complex mixture of polyphenols, with antioxidant activity. Numerous studies have been focused on the protective and preventing effect of this antioxidant activity on certain degenerative illnesses such as cardiovascular, cancer, and neurological diseases, cataracts and oxidative stress dysfunctions. The objective of this work was to evaluate total polyphenol content and antioxidant activity of several seeds, nuts, or grains such as Theobroma cacao, Canpsiandra comosa Benth (chiga), Sorghum bicolor, L. Moench, Melicoccus bijugatus (genip). Total polyphenol content was assessed by the Folin-Ciocalteau method and the antioxidant activity by the beta carotene/linoleate, reducing power, and the anti-radical activity methods. Results showed genip pericarp with the lowest polyphenol content (1.40 gGAE/100 g), and cacao beans with the highest (6.66 gGAE/100 g). Reducing power of cacao beans was also the highest and similar to the reducing power of 5.80 g ascorbic acid/100 g, followed by Campsiandra comosa. Moreover, Campsiandra comosa and cacao seeds presented an antioxidant activity comparable to that of the butylhydroxianisol, a synthetic antioxidant. The highest anti-radical activity was shown by Campsiandra comosa with an EC50 of 2.67 g/gDPPH. Total polyphenol content shows a good correlation with the antioxidant activity. Moreover, these seeds might have the same health beneficial effects attributed to other fruits and vegetables.
植物源食物不仅为人类饮食提供了某些抗氧化维生素(维生素C、维生素E和β-胡萝卜素),还提供了具有抗氧化活性的复杂多酚混合物。众多研究聚焦于这种抗氧化活性对某些退行性疾病(如心血管疾病、癌症、神经疾病、白内障和氧化应激功能障碍)的保护和预防作用。本研究的目的是评估几种种子、坚果或谷物(如可可、南美可可豆、高粱、人心果)的总多酚含量和抗氧化活性。采用福林-酚法评估总多酚含量,采用β-胡萝卜素/亚油酸法、还原能力法和抗自由基活性法评估抗氧化活性。结果显示,人心果果皮的多酚含量最低(1.40 g没食子酸当量/100 g),可可豆的多酚含量最高(6.66 g没食子酸当量/100 g)。可可豆的还原能力也最高,与5.80 g抗坏血酸/100 g的还原能力相似,其次是南美可可豆。此外,南美可可豆和可可种子的抗氧化活性与合成抗氧化剂丁基羟基茴香醚相当。南美可可豆的抗自由基活性最高,其对二苯代苦味酰基自由基(DPPH)的半数有效浓度(EC50)为2.67 g/g。总多酚含量与抗氧化活性呈现良好的相关性。此外,这些种子可能具有与其他水果和蔬菜相同的健康益处。