Xu Bingsen, Zhang Kexiong, Huang Yingqun
RNA. 2009 Mar;15(3):357-61. doi: 10.1261/rna.1368009. Epub 2009 Jan 15.
Lin28 is highly expressed in human and mouse embryonic stem (ES) cells. Here, we show that in mouse ES cells, specific repression of Lin28 results in decreased cell proliferation, while overexpression of Lin28 accelerates cell proliferation. Further, Lin28 associates specifically with ribonucleoprotein particles containing mRNAs for cyclins A and B and cdk4. Importantly, changes in Lin28 levels lead to corresponding changes in the levels of these proteins, and sequences from the 3' untranslated regions of cyclin B and cdk4 mRNAs exhibit stimulatory effects on translation of reporter genes in a Lin28-dependent fashion. Thus, we postulate that Lin28 may play a role in the regulation of translation of genes important for the growth and maintenance of pluripotent cells.
Lin28在人和小鼠胚胎干细胞（ES细胞）中高度表达。在此，我们表明在小鼠ES细胞中，Lin28的特异性抑制导致细胞增殖减少，而Lin28的过表达加速细胞增殖。此外，Lin28特异性地与含有细胞周期蛋白A、B和cdk4的mRNA的核糖核蛋白颗粒结合。重要的是，Lin28水平的变化导致这些蛋白质水平的相应变化，并且细胞周期蛋白B和cdk4 mRNA的3'非翻译区序列以Lin28依赖的方式对报告基因的翻译表现出刺激作用。因此，我们推测Lin28可能在调节对多能细胞的生长和维持重要的基因的翻译中发挥作用。
