基于流动注射的抗氧化能力快速筛选方法。

Flow injection based methods for fast screening of antioxidant capacity.

作者信息

Magalhães Luís M, Santos Miguel, Segundo Marcela A, Reis Salette, Lima José L F C

机构信息

REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal.

出版信息

Talanta. 2009 Mar 15;77(5):1559-66. doi: 10.1016/j.talanta.2008.10.034. Epub 2008 Oct 31.

DOI:10.1016/j.talanta.2008.10.034

PMID:19159764

Abstract

The role and importance of antioxidants in different fields, ranging from physiology to food technology, have become evident in the past years, requiring adequate analytical methodologies. Therefore, the determination of antioxidant capacity as a routine or screening analysis fosters its automation. In this context, several flow injection methods based on scavenging of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (ABTS(+)) or 2,2-diphenyl-1-picrylhydrazyl radical (DDPH) or based on the determination of total reducing capacity have been proposed. The objective of the present review is to critically compare the different approaches, regarding their degree of automation, their performance vs. the respective batch procedure and its applicability to real samples.

摘要

在过去几年中，抗氧化剂在从生理学到食品技术等不同领域的作用和重要性已变得显而易见，这就需要适当的分析方法。因此，将抗氧化能力测定作为常规或筛选分析促进了其自动化。在这种背景下，已经提出了几种基于清除2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）阳离子自由基（ABTS(+)）或2,2-二苯基-1-苦基肼自由基（DPPH）或基于总还原能力测定的流动注射方法。本综述的目的是严格比较不同方法，涉及它们的自动化程度、与各自批次程序相比的性能以及对实际样品的适用性。