触珠蛋白和血清淀粉样蛋白A作为动脉粥样硬化性血栓形成性缺血性中风诊断确认生物标志物的效用。
Usefulness of haptoglobin and serum amyloid A proteins as biomarkers for atherothrombotic ischemic stroke diagnosis confirmation.
作者信息
Brea David, Sobrino Tomás, Blanco Miguel, Fraga Máximo, Agulla Jesús, Rodríguez-Yáñez Manuel, Rodríguez-González Raquel, Pérez de la Ossa Natalia, Leira Rogelio, Forteza Jerónimo, Dávalos Antonio, Castillo José
机构信息
Department of Neurology, Hospital Clínico Universitario, University of Santiago de Compostela, Spain.
出版信息
Atherosclerosis. 2009 Aug;205(2):561-7. doi: 10.1016/j.atherosclerosis.2008.12.028. Epub 2008 Dec 30.
OBJECTIVE
To identify protein biomarkers in order to classify ischemic stroke subtypes using proteomic analysis and immunoenzymatic tools for clinical validation.
METHODS AND RESULTS
We performed a proteomic analysis in serum samples of 24 patients with ischemic stroke (12 atherothrombotic patients and 12 cardioembolic patients). In this study, based on two-dimensional electrophoresis and mass spectrometry we found four spots whose expression intensity was at least four times stronger in atherothrombotic patients than in cardioembolic patients. These spots were identified as haptoglobin related protein, serum amyloid A (two spots) and haptoglobin alpha chain. We validated the possible value of haptoglobin and serum amyloid A in a larger series of patients (n=262) with ischemic stroke using ELISA techniques. Haptoglobin levels >1040microg/mL identified atherothrombotic patients with 95% sensitivity and 88% specificity whereas serum amyloid A levels >160microg/mL identified atherothrombotic patients with 91% sensitivity and 83% specificity.
CONCLUSIONS
Haptoglobin and serum amyloid A are useful biomarkers for atherothrombotic ischemic stroke diagnosis confirmation.
目的
通过蛋白质组学分析和免疫酶学工具进行临床验证，以识别蛋白质生物标志物，从而对缺血性中风亚型进行分类。
方法与结果
我们对24例缺血性中风患者（12例动脉粥样硬化血栓形成患者和12例心源性栓塞患者）的血清样本进行了蛋白质组学分析。在本研究中，基于二维电泳和质谱分析，我们发现有4个斑点在动脉粥样硬化血栓形成患者中的表达强度至少是心源性栓塞患者的4倍。这些斑点被鉴定为触珠蛋白相关蛋白、血清淀粉样蛋白A（2个斑点）和触珠蛋白α链。我们使用ELISA技术在更大系列的缺血性中风患者（n = 262）中验证了触珠蛋白和血清淀粉样蛋白A的可能价值。触珠蛋白水平>1040μg/mL可识别动脉粥样硬化血栓形成患者，灵敏度为95%，特异性为88%；而血清淀粉样蛋白A水平>160μg/mL可识别动脉粥样硬化血栓形成患者，灵敏度为91%，特异性为83%。
结论
触珠蛋白和血清淀粉样蛋白A是用于确诊动脉粥样硬化血栓形成性缺血性中风的有用生物标志物。
Zotero 插件