利用相干反斯托克斯拉曼散射（CARS）显微镜对隐孢子虫卵囊进行化学特异性成像。
Chemically specific imaging of cryptosporidium oocysts using coherent anti-Stokes Raman scattering (CARS) microscopy.
作者信息
Murugkar S, Evans C L, Xie X S, Anis H
机构信息
School of Information Technology and Engineering (SITE), University of Ottawa, 800 King Edward, P.O. Box 450, Stn A, Ottawa, Ontario K1N6N5, Canada.
出版信息
J Microsc. 2009 Feb;233(2):244-50. doi: 10.1111/j.1365-2818.2009.03114.x.
Abstract
We demonstrate the application of coherent anti-Stokes Raman scattering microscopy for the rapid, label-free chemical imaging of waterborne pathogens. Chemically selective images of cryptosporidium were acquired in just a few seconds using coherent anti-Stokes Raman scattering microscopy, demonstrating its capability for the rapid detection of cryptosporidium at the single oocyst level. We discuss the applicability of such a technique in a near-real time automated water testing system.
摘要
我们展示了相干反斯托克斯拉曼散射显微镜在水性病原体快速、无标记化学成像中的应用。使用相干反斯托克斯拉曼散射显微镜在短短几秒钟内就获得了隐孢子虫的化学选择性图像，证明了其在单卵囊水平快速检测隐孢子虫的能力。我们讨论了这种技术在近实时自动水检测系统中的适用性。
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