怀孕是否会对龈下微生物群产生影响？

Does pregnancy have an impact on the subgingival microbiota?

作者信息

Adriaens Laurence M, Alessandri Regina, Spörri Stefan, Lang Niklaus P, Persson G Rutger

机构信息

Department of Periodontology, Division of Oral Microbiology, University of Bern, Bern, Switzerland.

出版信息

J Periodontol. 2009 Jan;80(1):72-81. doi: 10.1902/jop.2009.080012.

DOI:10.1902/jop.2009.080012

PMID:19228092

Abstract

BACKGROUND

We investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.

METHODS

Bacterial samples from weeks 12, 28, and 36 of pregnancy and at 4 to 6 weeks postpartum were processed for 37 species by checkerboard DNA-DNA hybridization. Clinical periodontal data were collected at week 12 and at 4 to 6 weeks postpartum, and bleeding on probing (BOP) was recorded at sites sampled at the four time points.

RESULTS

The mean BOP at week 12 and postpartum was 40.1% +/- 18.2% and 27.4% +/- 12.5%, respectively. The corresponding mean BOP at microbiologic test sites was 15% (week 12) and 21% (postpartum; not statistically significant). Total bacterial counts decreased between week 12 and postpartum (P <0.01). Increased bacterial counts over time were found for Neisseria mucosa (P <0.001). Lower counts (P <0.001) were found for Capnocytophaga ochracea, Capnocytophaga sputigena, Eubacterium saburreum, Fusobacterium nucleatum naviforme, Fusobacterium nucleatum polymorphum, Leptotrichia buccalis, Parvimonas micra (previously Peptostreptococcus micros or Micromonas micros), Prevotella intermedia, Prevotella melaninogenica, Staphylococcus aureus, Streptococcus anginosus, Streptococcus intermedius, Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis, Selenomonas noxia, and Veillonella parvula. No changes occurred between weeks 12 and 28 of pregnancy. Counts of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), and Treponema denticola did not change. Counts of P. gingivalis and T. forsythia at week 12 were associated with gingivitis (P <0.001).

CONCLUSIONS

Subgingival levels of bacteria associated with periodontitis did not change. P. gingivalis and T. forsythia counts were associated with BOP at week 12. A decrease was found in 17 of 37 species from week 12 to postpartum. Only counts of N. mucosa increased.

摘要

背景

我们调查了20名年龄≥18岁且未接受牙科护理的连续孕妇在孕期的临床和龈下微生物学变化。

方法

对妊娠第12周、28周和36周以及产后4至6周的细菌样本进行棋盘式DNA-DNA杂交，检测37种细菌。在妊娠第12周和产后4至6周收集临床牙周数据，并记录四个时间点采样部位的探诊出血（BOP）情况。

结果

妊娠第12周和产后的平均BOP分别为40.1%±18.2%和27.4%±12.5%。微生物检测部位相应的平均BOP分别为15%（第12周）和21%（产后；无统计学意义）。从第12周到产后，细菌总数减少（P<0.01）。黏膜奈瑟菌的细菌数量随时间增加（P<0.001）。微黄二氧化碳嗜纤维菌、生痰二氧化碳嗜纤维菌、沙氏真杆菌、舟形核梭杆菌、多形核梭杆菌、颊纤毛菌、微小单胞菌（以前称为微小消化链球菌或微小微单胞菌）、中间普氏菌、产黑色素普氏菌、金黄色葡萄球菌、咽峡炎链球菌、中间链球菌、变形链球菌、口腔链球菌、血链球菌、有害月形单胞菌和小韦荣球菌的数量减少（P<0.001）。妊娠第12周和28周之间无变化。伴放线聚集杆菌（以前称为伴放线放线杆菌）、牙龈卟啉单胞菌、福赛坦纳菌（以前称为福赛拟杆菌）和具核梭杆菌的数量没有变化。第12周时牙龈卟啉单胞菌和福赛坦纳菌的数量与牙龈炎有关（P<0.001）。