薏苡仁 testa 及其酚类成分对 Cu2+ 处理的低密度脂蛋白（LDL）氧化和脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞炎症的影响。

Effect of Adlay ( Coix lachryma-jobi L. var. ma-yuen Stapf) Testa and its phenolic components on Cu2+-treated low-density lipoprotein (LDL) oxidation and lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages.

作者信息

Huang Din-Wen, Kuo Yueh-Hsiung, Lin Fang-Yi, Lin Yun-Lian, Chiang Wenchang

机构信息

Graduate Institute of Food Science and Technology, Center for Food and Biomolecules, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan, Republic of China.

出版信息

J Agric Food Chem. 2009 Mar 25;57(6):2259-66. doi: 10.1021/jf803255p.

DOI:10.1021/jf803255p

PMID:19243096

Abstract

The aims of this study were to investigate the effects of adlay testa (AT) on Cu(2+)-treated low-density lipoprotein (LDL) oxidation, 2,2'-diphenyl-1-picrylhydrazyl (DPPH)-scavenging capacity, and lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages and determine its active components. The AT ethanolic extract (ATE) was partitioned into four fractions by various solvents as follows: n-hexane (ATE-Hex), ethyl acetate (ATE-Ea), n-butanol (ATE-Bu), and water (ATE-H(2)O). ATE-Ea and ATE-Bu were further fractionated into ATE-Ea-a-ATE-Ea-h and ATE-Bu-A-ATE-Bu-F, respectively, by column chromatography. Results showed that ATE-Ea, ATE-Bu, ATE-Ea-e, and ATE-Bu-C expressed antiradical, antioxidative, and anti-inflammatory activities with respect to the DPPH-scavenging capacity, LDL protection effect, and nitric oxide (NO) inhibitory activity. Inflammation was further modulated by ATE-Ea, ATE-Bu, ATE-Ea-e, and ATE-Bu-C through downregulating the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) proteins. The following components were found in ATE-Ea-e and ATE-Bu-C after purification and high-performance liquid chromatographic analysis: chlorogenic acid (CGA), vanillic acid (VA), caffeic acid (CA), p-coumaric acid (PCA), ferulic acid (FA), and 2-O-beta-glucopyranosyl-7-methoxy-4((2)H)-benzoxazin-3-one (GMBO). Results showed that CGA, CA, and FA were the major components responsible for the antioxidative and anti-inflammatory activities of ATE-Ea-e and ATE-Bu-C. Subsequently, each gram of ATE-Bu-C had 30.3 mg of CGA, 9.02 mg of CA, and 189 mg of GMBO, while each gram of ATE-Ea-e had 1.31 mg of VA, 3.89 mg of PCA, and 47.6 microg of FA. In conclusion, ATE has antioxidative and anti-inflammatory activities, and its effects are partially related to its phenolic components. Thus, ATE has the potential to be developed as a functional food targeting chronic diseases.

摘要

本研究旨在探讨薏苡仁 testa（AT）对 Cu(2+)处理的低密度脂蛋白（LDL）氧化、2,2'-二苯基-1-苦基肼（DPPH）清除能力以及脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞炎症的影响，并确定其活性成分。将 AT 乙醇提取物（ATE）用各种溶剂分为四个部分：正己烷（ATE-Hex）、乙酸乙酯（ATE-Ea）、正丁醇（ATE-Bu）和水（ATE-H₂O）。通过柱色谱法将 ATE-Ea 和 ATE-Bu 进一步分别分离为 ATE-Ea-a - ATE-Ea-h 和 ATE-Bu-A - ATE-Bu-F。结果表明，ATE-Ea、ATE-Bu、ATE-Ea-e 和 ATE-Bu-C 在 DPPH 清除能力、LDL 保护作用和一氧化氮（NO）抑制活性方面表现出抗自由基、抗氧化和抗炎活性。ATE-Ea、ATE-Bu、ATE-Ea-e 和 ATE-Bu-C 通过下调诱导型一氧化氮合酶（iNOS）和环氧化酶 2（COX-2）蛋白的表达进一步调节炎症。经过纯化和高效液相色谱分析后，在 ATE-Ea-e 和 ATE-Bu-C 中发现了以下成分：绿原酸（CGA）、香草酸（VA）、咖啡酸（CA）、对香豆酸（PCA）、阿魏酸（FA）和 2-O-β-吡喃葡萄糖基-7-甲氧基-4((2)H)-苯并恶嗪-3-酮（GMBO）。结果表明，CGA、CA 和 FA 是 ATE-Ea-e 和 ATE-Bu-C 抗氧化和抗炎活性的主要成分。随后，每克 ATE-Bu-C 含有 30.3 毫克 CGA、9.02 毫克 CA 和 189 毫克 GMBO，而每克 ATE-Ea-e 含有 1.31 毫克 VA、3.89 毫克 PCA 和 47.6 微克 FA。总之，ATE 具有抗氧化和抗炎活性，其作用部分与其酚类成分有关。因此，ATE 有潜力被开发为一种针对慢性疾病的功能性食品。