白细胞介素-23和白细胞介素-17在强直性脊柱炎中的表达及白细胞介素-23对白细胞介素-17产生的影响
Expression of IL-23 and IL-17 and effect of IL-23 on IL-17 production in ankylosing spondylitis.
作者信息
Wang Xinwei, Lin Zhiming, Wei Qiujing, Jiang Yingjuan, Gu Jieruo
机构信息
Department of Rheumatology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, People's Republic of China.
出版信息
Rheumatol Int. 2009 Sep;29(11):1343-7. doi: 10.1007/s00296-009-0883-x. Epub 2009 Feb 27.
The objective of this study was to investigate the expression of IL-23 and IL-17 and the influence of IL-23 on IL-17 production in ankylosing spondylitis (AS) patients. IL-23 and IL-17 levels in the serum and supernatants of cultured peripheral blood mononuclear cells (PBMCs) were determined by ELISA. IL-23p19 mRNA expression in PBMCs were analyzed using RT-PCR. The patients with AS at active stage showed elevated levels of IL-23 and IL-17 in the serum and supernatants of cultured PBMCs. A higher expression of IL-23p19 mRNA in PBMCs of AS patients was also observed. A significantly enhanced production of IL-17 in the supernatants of cultured PBMCs was found in the presence of recombinant IL-23 and this effect was more significant in patients with AS. The results suggest that IL-23 and IL-17 may play critical roles in the pathogenesis of AS and IL-23-stimulated production of IL-17 by PBMCs may be responsible for the development of AS.
本研究的目的是调查强直性脊柱炎（AS）患者中白细胞介素-23（IL-23）和白细胞介素-17（IL-17）的表达情况以及IL-23对IL-17产生的影响。采用酶联免疫吸附测定法（ELISA）测定培养的外周血单个核细胞（PBMCs）的血清及上清液中IL-23和IL-17水平。运用逆转录聚合酶链反应（RT-PCR）分析PBMCs中IL-23p19信使核糖核酸（mRNA）的表达。活动期AS患者血清及培养的PBMCs上清液中IL-23和IL-17水平升高。同时观察到AS患者PBMCs中IL-23p19 mRNA表达较高。在重组IL-23存在的情况下，培养的PBMCs上清液中IL-17的产生显著增强，且这种效应在AS患者中更为明显。结果表明，IL-23和IL-17可能在AS发病机制中起关键作用，并且PBMCs受IL-23刺激产生IL-17可能与AS的发展有关。
Zotero 插件