Li Xu-Shan, Jiang Zhi-yong, Wang Fu-shengi, Ma Yun-Bao, Zhang Xue-mei, Chen Ji-jun
Department of Phanrmacy, Dali College, Dali 671003, China.
Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(23):2790-3.
To study the chemical constituents of Swertia mileensis.
The air-dried whole plants of S. mileensis were extracted with 50% EtOH. The EtOH extract was suspended in H20 and extracted with petroleum ether, CHCl3 and n-BuOH successively. The compounds were isolated and purified by column chromatography from the CHCl3 fraction, and identified based on spectral analyses (MS, H-NMR, 13C-NMR).
Twelve compounds were isolated from S. mileensis, and were elucidated as 1,5, 8-trihydroxy-3-methoxyxanthone (1), 1-hydroxyl-2, 3, 5, 7-tetramethoxyxanthone (2), 1-hydroxyl-3, 5, 8-trimethoxyxanthone (3), 1-hydroxyl-2, 3, 4, 6-tetramethoxyxanthone (4), 1-hydroxyl-2, 3, 4, 7-tetramethoxyxanthone (5), 1,8-dihydroxy-3, 5-dimethoxyxanthone (6), 1, 7-dihydroxy-3, 8-dimethoxyxanthone (7), 1, 3, 5, 8-tetrahydroxyxanthone (8), balanophonin (9), oleanolic acid (10), maslinic acid (11), sumaresinolic acid (12).
Compounds 1, 3, 7-9, 11 and 12 were obtained from S. mileensis for the first time.
研究青叶胆的化学成分。
将青叶胆的风干全草用50%乙醇提取。乙醇提取物悬浮于水中，依次用石油醚、三氯甲烷和正丁醇萃取。从三氯甲烷萃取物中通过柱色谱分离纯化化合物，并基于光谱分析（质谱、氢核磁共振、碳-13核磁共振）进行鉴定。
从青叶胆中分离出12个化合物，分别鉴定为1,5,8-三羟基-3-甲氧基呫吨酮（1）、1-羟基-2,3,5,7-四甲氧基呫吨酮（2）、1-羟基-3,5,8-三甲氧基呫吨酮（3）、1-羟基-2,3,4,6-四甲氧基呫吨酮（4）、1-羟基-2,3,4,7-四甲氧基呫吨酮（5）、1,8-二羟基-3,5-二甲氧基呫吨酮（6）、1,7-二羟基-3,8-二甲氧基呫吨酮（7）、1,3,5,8-四羟基呫吨酮（8）、巴兰诺芬宁（9）、齐墩果酸（10）、山楂酸（11）、苏门树脂酸（12）。
化合物1、3、7-9、11和12首次从青叶胆中获得。
