巴西围产期感染艾滋病毒-1亚型的儿童中的流行情况。

Prevalence of HIV-1 subtypes in Brazilian children with perinatally acquired infection.

作者信息

Molina Rosana M, Torina Anali G, Biffi Keila, Bismara Beatriz A P, Albuquerque Dulcineia M, Andrade Paula D, Anjos Emanuel B V, Toro Adleia D C, Nolasco Marcos T, Vilela Maria M S, Costa Sandra C B

机构信息

Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.

出版信息

J Int Assoc Physicians AIDS Care (Chic). 2009 Mar-Apr;8(2):106-12. doi: 10.1177/1545109709331808. Epub 2009 Mar 4.

DOI:10.1177/1545109709331808

PMID:19264724

Abstract

HIV-1 infection has increased among women in recent years. The HIV-1 env gene (structural gene) has the greatest variation in all the HIV gene regions. In this study, 58 samples from infants infected with HIV-1 via perinatal transmission were analyzed. All the 58 samples were submitted to Nested-polymerase chain reaction of the env gene region for posterior viral genotyping using EN 70 and EN 85 (first polymerase chain reaction) and EN 80 and EN 95 (second polymerase chain reaction) primers, with the product of the 682 base pair amplification. After Nested-polymerase chain reaction for genotyping, purification of the product, and direct sequencing in a MegaBace 1000 automatic sequencer, 56 genotypes were found in the 58 HIV-1-positive children of the study, where 47 (83.93%) were HIV-1 subtype B infected and 9 (16.07%) were HIV-1 subtype F1 infected. The results demonstrate the predominance of subtype B followed by subtype F in Southeast Brazil.

摘要

近年来，女性中的HIV-1感染率有所上升。HIV-1 env基因（结构基因）在所有HIV基因区域中变异最大。在本研究中，对58例经围产期传播感染HIV-1的婴儿样本进行了分析。所有58个样本均采用EN 70和EN 85（第一次聚合酶链反应）以及EN 80和EN 95（第二次聚合酶链反应）引物，对env基因区域进行巢式聚合酶链反应，以进行后续病毒基因分型，扩增产物为682个碱基对。在进行基因分型的巢式聚合酶链反应、产物纯化以及在MegaBace 1000自动测序仪中进行直接测序后，在该研究的58名HIV-1阳性儿童中发现了56种基因型，其中47例（83.93%）感染了HIV-1 B亚型，9例（16.07%）感染了HIV-1 F1亚型。结果表明，在巴西东南部，B亚型占主导地位，其次是F亚型。