Lilleri Daniele, Zelini Paola, Fornara Chiara, Comolli Giuditta, Revello Maria Grazia, Gerna Giuseppe
Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Clin Immunol. 2009 Jun;131(3):395-403. doi: 10.1016/j.clim.2009.02.002. Epub 2009 Mar 5.
The kinetics of primary human cytomegalovirus (HCMV) infection and specific T-cell responses were investigated in 16 immunocompetent pregnant women and 8 solid-organ transplant recipients (SOTR). T-cell responses to whole HCMV and to pp65 and IE-1 peptides were determined by flow cytometry evaluation of IFNgamma production. HCMV-specific CD4(+) and CD8(+) T-cells appeared earlier and simultaneously in immunocompetent subjects, whereas specific CD8(+) T-cells preceded CD4(+) T-cells in half of the SOTR examined. The magnitude of the HCMV-specific T-cell pool was comparable. HCMV load reached peak levels 100-1000 times higher in SOTR than in immunocompetent women, while the virus persisted for months in blood of both groups. T-cells directed to pp65 and IE-1 were only detected in a portion of subjects developing a full T-cell response to the whole virus. Thus, the development of cell-mediated immune response in primary HCMV infection may be missed when looking at pp65 and IE-1 peptide-stimulated T-cells only.
在16名免疫功能正常的孕妇和8名实体器官移植受者（SOTR）中研究了原发性人巨细胞病毒（HCMV）感染的动力学和特异性T细胞反应。通过对IFNγ产生的流式细胞术评估来确定对完整HCMV以及pp65和IE-1肽的T细胞反应。HCMV特异性CD4(+)和CD8(+) T细胞在免疫功能正常的受试者中更早且同时出现，而在一半接受检查的SOTR中，特异性CD8(+) T细胞先于CD4(+) T细胞出现。HCMV特异性T细胞库的大小相当。SOTR中的HCMV载量达到的峰值水平比免疫功能正常的女性高100 - 1000倍，而病毒在两组的血液中都持续存在数月。仅在一部分对完整病毒产生完全T细胞反应的受试者中检测到针对pp65和IE-1的T细胞。因此，仅观察pp65和IE-1肽刺激的T细胞时，可能会错过原发性HCMV感染中细胞介导免疫反应的发展。
