Kathiresan K, Manivannan S, Nabeel M A, Dhivya B
Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2009 Jun 1;71(1):133-7. doi: 10.1016/j.colsurfb.2009.01.016. Epub 2009 Jan 31.
In this work, in vitro biosynthesis of silver nanoparticles was achieved using AgNO(3) as a substrate by Penicillium fellutanum isolated from coastal mangrove sediment. The biosynthesis was faster within minutes of silver ion coming in contact with the cell filtrate. Presence of silver nanoparticles in the culture filtrate was confirmed by absorption peak at 430 nm, as well under transmission electron microscope. The biosynthesis of nanoparticles was the maximum when the culture filtrate was treated with 1.0 mM AgNO(3), maintained at 0.3% NaCl and pH 6.0, incubated at 5 degrees C for 24h. The culture filtrate, precipitated with ammonium sulphate, was proved to have a single protein band with a molecular weight of 70 kDa using polyacrylamide gel electrophoresis. The present work highlighted the possibility of using the marine fungal strain of P. fellutanum to achieve a fast rate of nanoparticles synthesis.
在这项工作中，从沿海红树林沉积物中分离出的费氏青霉（Penicillium fellutanum）以硝酸银（AgNO₃）为底物实现了银纳米颗粒的体外生物合成。银离子与细胞滤液接触后几分钟内，生物合成速度加快。通过在430nm处的吸收峰以及透射电子显微镜也证实了培养滤液中存在银纳米颗粒。当培养滤液用1.0 mM硝酸银处理，保持在0.3%氯化钠和pH 6.0条件下，于5℃孵育24小时时，纳米颗粒的生物合成量最大。使用聚丙烯酰胺凝胶电泳证明，用硫酸铵沉淀的培养滤液有一条分子量为70 kDa的单一蛋白带。目前的工作突出了利用费氏青霉这种海洋真菌菌株实现快速合成纳米颗粒的可能性。
