使用高速逆流色谱法和高效液相色谱法从蜜树茶（Cyclopia subternata）中分离异芒果苷。
Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.
作者信息
de Beer Dalene, Jerz Gerold, Joubert Elizabeth, Wray Victor, Winterhalter Peter
机构信息
ARC Infruitec-Nietvoorbij, Stellenbosch, South Africa.
出版信息
J Chromatogr A. 2009 May 8;1216(19):4282-9. doi: 10.1016/j.chroma.2009.02.056. Epub 2009 Feb 24.
Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy.
异芒果苷是从南非蜜环菌中通过多步过程分离得到的，该过程包括提取、液液分配、高速逆流色谱法（HSCCC）和半制备反相高效液相色谱法（HPLC）。使用乙酸乙酯 - 甲醇 - 水（1:1:2，v/v）进行液液分配，对南非蜜环菌叶甲醇提取物中的酚类化合物进行富集。使用由叔丁基甲基醚 - 正丁醇 - 乙腈 - 水（3:1:1:5，v/v）组成的三元溶剂系统，通过HSCCC对富集部分进一步分离。通过半制备反相HPLC从主要含有芒果苷和异芒果苷的部分中分离出异芒果苷。异芒果苷的化学结构通过液相色谱 - 高分辨率电喷雾电离质谱以及一维和二维核磁共振光谱得以确认。
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