一种用于验证鼠疫耶尔森菌候选菌株中是否不存在pgm基因座以获得选择剂豁免的策略。
A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption.
作者信息
Jenkins Amy L, Worsham Patricia L, Welkos Susan L
机构信息
Bacteriology Division, U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA.
出版信息
J Microbiol Methods. 2009 Jun;77(3):316-9. doi: 10.1016/j.mimet.2009.02.013. Epub 2009 Mar 10.
Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm(-) strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y. pestis strains being considered as candidates for select agent exemption by PCR analysis of virulence-associated genes.
根据联邦法规，鼠疫耶尔森菌是美国卫生与公众服务部指定的生物制剂。某些减毒的鼠疫耶尔森菌菌株，如pgm（-）菌株，不受这些法规的限制。在此，我们描述了一种策略，通过对毒力相关基因进行PCR分析，来验证被视为可能获得指定生物制剂豁免资格的鼠疫耶尔森菌菌株中是否不存在pgm基因座。
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